Addgene

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pHHYTK113

Plasmid

#200144

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

sfGFP1-10

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2-QUAS:GCaMP7a

Plasmid

#184817

Purpose

Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GCaMP7a-pA

Use

Zebrafish tol2 transgenesis

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV5

Plasmid

#78629

Purpose

GESTALT V5 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V5

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-EGFP-BarcodeV3

Plasmid

#78631

Purpose

GESTALT V3 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-EGFP-BarcodeV4

Plasmid

#78630

Purpose

GESTALT V4 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK122

Plasmid

#200146

Purpose

Type 3b part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

sfGFP1-10

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK101

Plasmid

#200131

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Su9-MTS (2-96)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK114

Plasmid

#200147

Purpose

Type 3 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

ymNeonGreen

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK160

Plasmid

#200154

Purpose

Type 4b part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

tT7

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK117

Plasmid

#200150

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

ymNeonGreen

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK116

Plasmid

#200149

Purpose

Type 3b part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

ymNeonGreen

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK125

Plasmid

#200137

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

OM-Tom70 (2-98)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK157

Plasmid

#200151

Purpose

Type 2 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

pSP6_Kozak

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK159

Plasmid

#200153

Purpose

Type 4 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

tT7

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK109

Plasmid

#200135

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

BPSV40-NLS

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK107

Plasmid

#200133

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Kar2-HDEL

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK110

Plasmid

#200141

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

sfGFP11

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK126

Plasmid

#200138

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Pot1-PTS2 (2-50)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK106

Plasmid

#200132

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Kar2-SS

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK108

Plasmid

#200134

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

BPSV40-NLS

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK111

Plasmid

#200142

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

sfGFP11

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK124

Plasmid

#200136

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

IMS-Mia40 (2-70)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK188

Plasmid

#200156

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

6xHis

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK158

Plasmid

#200152

Purpose

Type 2 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

pT7_LacO

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK115

Plasmid

#200148

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

ymNeonGreen

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK161

Plasmid

#200155

Purpose

Type 67 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

LacI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK121

Plasmid

#200145

Purpose

Type 3 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

sfGFP1-10

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHYTK112

Plasmid

#200143

Purpose

Type 3a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

sfGFP1-10

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA70

Plasmid

#174784

Purpose

RNAi

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

Intron-containing hairpin RNA (ihpRNA)-NbJIM2

Use

RNAi

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGW-GOI-TurboID

Plasmid

#209378

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGW-TurboID-GOI

Plasmid

#209380

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGW-GFP-TurboID-GOI

Plasmid

#209381

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGW-GOI-TurboID-GFP

Plasmid

#209379

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLRD16

Plasmid

#208268

Purpose

Production of syn-stemar-13-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL8

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCYP64

Plasmid

#212897

Purpose

Expresses the Priestia megaterium LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

P450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…

Promoter

PcymRC

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pZY101

Plasmid

#73932

Purpose

This binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.html

Depositor

Zhanyuan Zhang

Article

Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

Insert

bar

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGW-TurboID-GOI-GFP

Plasmid

#209383

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA72

Plasmid

#174783

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_XopJ4, Kmr plant selection marker

Use

Tags

Expression

Plant

Mutation

WT + STOP codon

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGW-GFP-GOI-TurboID

Plasmid

#209382

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pB_35S/mEGFP

Plasmid

#135320

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB3C5-proD-B0032-E0051

Plasmid

#107241

Purpose

Expresses lacZa-GFP fusion from constitutive proD promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProA-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proD

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB3C5-proA-B0032-E0051

Plasmid

#107244

Purpose

Expresses lacZa-GFP fusion from constitutive proA promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProA-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proA

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA57

Plasmid

#174770

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-4xMyc, Kmr plant selection marker

Use

Tags

4xMyc

Expression

Plant

Mutation

WT

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA71

Plasmid

#174785

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker

Use

Tags

3xFLAG

Expression

Plant

Mutation

WT

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA68

Plasmid

#174781

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V-eGFP, Kmr plant selection marker

Use

Tags

eGFP

Expression

Plant

Mutation

L17E/D481V

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA66

Plasmid

#174779

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V-eGFP, Kmr plant selection marker

Use

Tags

eGFP

Expression

Plant

Mutation

D481V

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA55

Plasmid

#174768

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E without STOP codon

Use

Unspecified

Tags

Expression

Mutation

L17E

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA56

Plasmid

#174769

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V without STOP codon

Use

Unspecified

Tags

Expression

Mutation

L17E/D481V

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA54

Plasmid

#174767

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V without STOP codon

Use

Unspecified

Tags

Expression

Mutation

D481V

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZA53

Plasmid

#174766

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn without STOP codon

Use

Unspecified

Tags

Expression

Mutation

WT

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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