1. Myr-(iSH2-p85)-p110alpha-Myc

   Plasmid

   #1410

   Depositor

   Lewis Cantley

   Insert

   PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha promoter

   Available since

   June 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. Dcp1

   Plasmid

   #201410

   Purpose

   Bacterial protein expression of Dcp1

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Dcp1 (DCP1 Budding Yeast)
   

   Use

   Tags

   MBP, TEV and TEV, 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-flex-rev-g2-2A-Venus

   Plasmid

   #71410

   Purpose

   This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.

   Depositor

   William Wisden

   Article

   Uygun et al J Neurosci. 2016 Nov 2;36(44):11171-1118

   Inserts

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse hdc gene promoter, works as a pan neuronal …

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP

   Plasmid

   #114106

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al J Neurosci Res. 2019 Apr 7. doi: 10.1002

   Insert

   Gaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-IC2-N237

   Plasmid

   #51410

   Purpose

   expression of GFP-tagged N-terminal AA 1-237 of dynein intermediate chain IC2C in mammalian cells

   Depositor

   Trina Schroer

   Article

   King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

   Insert

   dynein intermediate chain IC2C (Dync1i2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   AA 1-237

   Promoter

   CMV

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pOPINK-OTUD2 (OTU, aa 132-314)

   Plasmid

   #61410

   Purpose

   Expresses human OTUD2 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD2 (YOD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-131 and aa 315-348.

   Promoter

   T7

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCu-GFP-Atg17(416) (L105D)

   Plasmid

   #121410

   Purpose

   Expressing GFP-Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-GFP-ATG17 ORF-CYC1 terminator
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Leucine 105 to Aspartic acid

   Promoter

   CUP1 promoter

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)

   Plasmid

   #114102

   Purpose

   fusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

   Insert

   GLucM23-iChloC-EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

   Plasmid

   #114101

   Purpose

   fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

   Insert

   Gaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-sbGLuc-CheRiff-EGFP

   Plasmid

   #114108

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.

   Insert

   Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA 3.1 - circMAN2A1

    Plasmid

    #198837

    Purpose

    This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

    Depositor

    Stefan Stamm

    Article

    Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

    Insert

    circular mannosidase alpha class 2A member 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    circular RNA composed by 2 exons

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

    Plasmid

    #214102

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-EGFR-L858R-T790M-dual-epegRNA

    Plasmid

    #214100

    Purpose

    Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR L858R epegRNA/EGFR T790M epegRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114103

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi

    Insert

    sbGLuc-VChR1-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    motoneuron-specific Homeobox 9 promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_GRB2-2/2

    Plasmid

    #91410

    Purpose

    Protein expression and purification of human SH3 domain construct GRB2-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GRB2-2/2 (GRB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lenti-EGFR-L858R-T790M-dual-nick sgRNA

    Plasmid

    #214101

    Purpose

    Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114104

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    sbGLuc-VChR1-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-SLC7A11_STOP

    Plasmid

    #161410

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

    Plasmid

    #114100

    Purpose

    fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221-BSX

    Plasmid

    #101410

    Purpose

    Donor Vector containing BSX transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    BSX (BSX Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223_GATA3_p.R330K

    Plasmid

    #81410

    Purpose

    Gateway Donor vector containing GATA3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    GATA3 (GATA3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    V3A; R330K

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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