Addgene

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pGEX-5x3-hMeCP2-delN256-G273X

Plasmid

#48090

Purpose

Bacterial expression of GST tagged AT-Hook2-G273X

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted the N-terminal 256 codons, expresses AT-H…

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-5x3-hMeCP2-delN256-R270X

Plasmid

#48089

Purpose

Bacterial expression of GST tagged AT-Hook2-R270X

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted the N-terminal 256 codons, expresses AT-H…

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-iRFP713(V256C)-P2A-EGFP

Plasmid

#198071

Purpose

The AAV vector for iRFP713(V256C) and EGFP

Depositor

Michiyuki Matsuda

Insert

iRFP713(V256C)

Use

AAV

Tags

P2A-EGFP

Expression

Mutation

V256C mutation

Promoter

CMV

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(D256A)-IRES-puro

Plasmid

#128677

Purpose

mammalian expression of Flag-Polß(D256A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D256A

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

Plasmid

#198069

Purpose

Mammalian expression of iRFP713(C15S/V256C) and EGFP

Depositor

Michiyuki Matsuda

Insert

iRFP713(C15S/V256C)

Use

Tags

P2A-mCherry

Expression

Mammalian

Mutation

C15S/V256C mutation

Promoter

CAG

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIbleo-iRFP713(C15S/V256C)-P2A-EGFP

Plasmid

#198062

Purpose

The lentiviral vector for iRFP713(C15S/V256C) and EGFP

Depositor

Michiyuki Matsuda

Insert

iRFP713(C15S/V256C)

Use

Lentiviral

Tags

P2A-EGFP

Expression

Mammalian

Mutation

C15S/V256C mutation

Promoter

CAG

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

Plasmid

#127789

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in His2…

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1969 - pAAV-AiE2563m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214597

Purpose

AiE2563m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1976 - pAAV-AiE2569m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214600

Purpose

AiE2569m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA

Plasmid

#108735

Purpose

Expresses N-terminus to aa256 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa256

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2603_pCAG-PV1-iCre-N256-L1-ABI-NLS-BGHpA

Plasmid

#108743

Purpose

Expresses N-terminus to aa256 of Cre recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa256

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1861 - pAAV-AiE2565m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214560

Purpose

AiE2565m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2562_pCAG-PV1-GAI-L1-VCre-173C-NLS-BGHpA

Plasmid

#108772

Purpose

Expresses aa173 to C-terminus of VCre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment aa173 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA

Plasmid

#108774

Purpose

Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment aa270 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2211_pCAG-PV1-iCre-N256-L1-GID1-NLS-BGHpA

Plasmid

#108727

Purpose

Expresses N-terminus to aa256 of Cre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa256

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1951 - pAAV-AiE2560m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230745

Purpose

AiE2560m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP20070 - pAAV-AiE2569m-minBG-iCre(R297T)-BGHpA

Plasmid

#230795

Purpose

AiE2569m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1902 - pAAV-AiE2561m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230733

Purpose

AiE2561m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP20025 - pAAV-AiE2564m-minBG-iCre(R297T)-BGHpA

Plasmid

#220733

Purpose

AiE2564m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Insert

iCre(R297T)

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1867 - pAAV-AiE2562m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#220676

Purpose

AiE2562m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge