We narrowed to 19,116 results for: INO

1. p15aC-4D1D-5

   Plasmid

   #107866

   Purpose

   Bacterial expression of PIS, PI4K and PI4P5K

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Inserts

   PIS
   phosphatidylinositol 4-phosphate 5-kinase
   phosphatidylinositol 4-kinase β
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   proD and proD (in operon)

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p15aC-3D1D-5

   Plasmid

   #121050

   Purpose

   Bacterial expression of PIS, PI4K and PI3P5K mutant E545K

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Inserts

   PIS
   phosphatidylinositol 4-phosphate 5-kinase
   phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K
   

   Use

   Tags

   myc and none

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAJE3-E7

   Plasmid

   #214359

   Purpose

   Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

   Depositor

   Ryan Mehl

   Article

   Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

   Inserts

   2nd generation Tet2 "E7" aminoacyl tRNA synthetase
   Lpp promoted M. jannaschii tRNA
   Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
   Orthogonal "D4II" ColE1 origin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AmpR and lpp

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. human rSHIP2 (18-893)

   Plasmid

   #128568

   Purpose

   Expression of human SHIP2 in bacteria

   Depositor

   Christophe Erneux

   Article

   Pesesse et al FEBS Lett. 1998 Oct 23;437(3):301-3.

   Insert

   SHIP2 (INPPL1 Human)
   

   Use

   Tags

   His Xpress (N-terminal)

   Expression

   Bacterial

   Mutation

   SHIP2 coding sequence from amino acid 18 (A) till…

   Promoter

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-pchD WT

   Plasmid

   #187973

   Purpose

   Expresses salicylate adenylation enzyme PchD from Pseudomonas aeruginosa

   Depositor

   Audrey Lamb

   Article

   Shelton et al J Biol Inorg Chem. 2022 May 5. pii: 10.1

   Insert

   pchD WT
   

   Use

   Tags

   Hexahistag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Combined γB2FL-FRET probe

   Plasmid

   #209094

   Purpose

   mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

   Depositor

   Takeharu Nagai

   Article

   Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

   Insert

   PcdhgB2 (Pcdhgb2 Mouse)
   

   Use

   Tags

   PA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Combined γB2ΔICD-FRET probe

   Plasmid

   #209093

   Purpose

   mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

   Depositor

   Takeharu Nagai

   Article

   Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

   Insert

   PcdhgB2 (Pcdhgb2 Mouse)
   

   Use

   Tags

   PA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET14b-PITPalpha

   Plasmid

   #58256

   Purpose

   bacterial expression of human PITP alpha

   Depositor

   Shamshad Cockcroft

   Article

   Hara et al J Biol Chem. 1997 Jun 6;272(23):14908-13

   Insert

   PITP alpha (PITPNA Human)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pNC2

   Plasmid

   #72438

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg

   Promoter

   T7 and n/a

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSG086

    Plasmid

    #214263

    Purpose

    PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)

    Depositor

    Ute Romling

    Article

    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

    Insert

    Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    substitution of 31 amino acids of FtsH2 (F124RRFA…

    Promoter

    T7 promoter

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBlueSKMimRNA

    Plasmid

    #102535

    Purpose

    Template for synthesis of Minos transposase mRNA

    Depositor

    Michalis Averof

    Article

    Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

    Insert

    Minos transposase mRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-RILPdel212N

    Plasmid

    #110499

    Purpose

    Dominant Negative

    Depositor

    Bernardo Mainou, Terence Dermody

    Article

    Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

    Insert

    RILPdel212N (RILP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino terminal 212 amino acids

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-PaODH

    Plasmid

    #110802

    Purpose

    Expresses PaODH and opine dehydrogenase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

    Insert

    Pa4835
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Human SHIP2

    Plasmid

    #124645

    Purpose

    Expresses HIS tagged human SHIP2 in COS-7/HEK cells

    Depositor

    Christophe Erneux

    Article

    Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-5

    Insert

    INPPL1 (INPPL1 Human)
    

    Use

    Tags

    His Xpress (N-terminal)

    Expression

    Mammalian

    Mutation

    coding sequence starts at amino acid 18 as calcul…

    Promoter

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PH-Akt-GFP

    Plasmid

    #51465

    Purpose

    Is a biosensor for PIP3/PI(3,4)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-NT*-HRV3CP

    Plasmid

    #162795

    Purpose

    Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

    Depositor

    Gottfried Otting

    Article

    Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

    Insert

    Human rhinovirus 14 3C protease
    

    Use

    Tags

    NT* solubility tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PH-PLCD1-GFP

    Plasmid

    #51407

    Purpose

    Is a biosensor for PI(4,5)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    PH-PLCD1 (aa 1–170) (PLCD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PH-Akt(R25C)-GFP

    Plasmid

    #51466

    Purpose

    Non-PIP3 binding control for PH-Btk-GFP

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R25C

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

19. p15aC-1D-5

    Plasmid

    #107865

    Purpose

    Bacterial expression of PIS and PI4P5K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    proD and proD (in operon)

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p15aC-4D1D

    Plasmid

    #107864

    Purpose

    Bacterial expression of PIS and PI4K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-kinase β
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    proD

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge