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pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

Plasmid

#111818

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

Mef2c (Mef2c Mouse)
Tdtomato
Gata4 (Gata4 Mouse)
Tbx5

Use

Cloning intermediate

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-rDA2m-IRES-EGFP-CAAX

Plasmid

#208694

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA2m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

Plasmid

#164612

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Expression

Mammalian

Mutation

S383T, F177A

Promoter

CMV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_CCK1.0

Plasmid

#208673

Purpose

Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0

Use

AAV

Promoter

hSyn

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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FlucWT-HA-GFP11-N1

Plasmid

#91954

Purpose

Expresses FlucWT-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucWT-HA-GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-YTHDC1-FL-mCherry-Cry2

Plasmid

#177124

Purpose

Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTHDC1 Human)

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

SFFV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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FlucDM-HA-GFP11-N1

Plasmid

#91956

Purpose

Expresses aggregation-prone FlucDM-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucDM-HA-GFP11

Expression

Mammalian

Mutation

R188Q,R261Q

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0med

Plasmid

#153286

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Has Service

AAV2

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

hSyn

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_-DIO-GRAB_NPY1.0

Plasmid

#208677

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0

Use

AAV

Promoter

EF1_

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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eTd-CBE

Plasmid

#196601

Purpose

Mammalian expression of eTd-CBE

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_DA1m

Plasmid

#113049

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

Depositor

Yulong Li

Article

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has Service

AAV9

Insert

GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)

Use

AAV

Promoter

hSyn

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_g5-HT3.0mut

Plasmid

#208712

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cells

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_DA1h

Plasmid

#113050

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons

Depositor

Yulong Li

Article

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has Service

AAV Retrograde and AAV9

Insert

GPCR activation based DA sensor GRAB_DA1h (DRD2 Human)

Use

AAV

Promoter

hSyn

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_NPY1.0

Plasmid

#208675

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pKnockinDonor-human-p16INK4A-P2A-mCherry

Plasmid

#137026

Purpose

donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

Depositor

Chunliang Li

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

Insert

p16INK4A-knockin-homology arm-P2A-mCherry

Tags

P2A-mCherry

Expression

Bacterial

Promoter

no promoter

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-EGFP-CAAX

Plasmid

#208685

Purpose

Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

membrane-localized enhanced green fluorescent protein EGFP-CAAX

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA2h

Plasmid

#208701

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3mut

Plasmid

#208704

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_gDA3mut in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) control sensor GRAB_gDA3mut

Use

AAV

Promoter

hSyn

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/Rabaptin-5:R5BD

Plasmid

#168808

Purpose

Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members

Depositor

Guangpu Li

Article

Qi et al Methods Mol Biol. 2015;1298:259-70. doi:

Insert

Rab5-binding domain (aa739-862) of Rabaptin-5

Tags

GST

Expression

Bacterial

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_eCB2.0

Plasmid

#164606

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

hSyn

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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