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RhoC FLARE.sc mCer, mVenus - CA

Plasmid

#65424

Purpose

RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Use

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, Q63L in RhoC

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
RhoC FLARE.sc mCer, mVenus - DN

Plasmid

#65423

Purpose

RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Use

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, T19N in RhoC

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
Lck-FRB-ECFP(W66A)

Plasmid

#67902

Purpose

Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

Lck-FRB-ECFP(W66A)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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FRB-ECFP(W66A)-Giantin

Plasmid

#67903

Purpose

Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

FRB-ECFP(W66A)-Giantin

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-AAVS1_sgRNA

Plasmid

#183891

Purpose

pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKCZeta KR HA Tagged

Plasmid

#24608

Depositor

Jeff Wrana

Article

Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Insert

PKCZeta (Prkcz Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

K281R mutation in the ATP binding domain causing …

Promoter

Available since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pEF-HA/Net1A

Plasmid

#28262

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1A (Net1 Mouse)

Use

Tags

2x HA

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
p63(1-29)-mCherry-N1

Plasmid

#67895

Purpose

Expresses of fusion of mCherry with the N-terminal 29 amino acids of p63RhoGEF

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

p63RhoGEF(1-29)-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEF-HA-Net1

Plasmid

#28264

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1 (Net1 Mouse)

Use

Tags

2x HA

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-KG/Net1

Plasmid

#28260

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1 (Net1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
pmCherry-C1-DH

Plasmid

#67898

Purpose

Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-DH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-FKBP12-C1-DH

Plasmid

#67901

Purpose

Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12-DH

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLVUT-eC3GFP

Plasmid

#225485

Purpose

Tet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFP

Depositor

Robert Gross

Article

Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

Insert

Exoenzyme C3 transferase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 30-251, See Depositor Comments

Promoter

Human ubiquitin C

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB

Plasmid

#225488

Purpose

Tet-on lentiviral vector for controlled expression of GDNF

Depositor

Robert Gross

Article

Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

Insert

Tetracycline-controlled transactivator fused with Krüppel-associated box (KRAB) domain following an internal ribosome entry site

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Mouse phosphoglycerate kinase 1

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-CfANLN_sgRNA

Plasmid

#183880

Purpose

pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-KG/Net1A

Plasmid

#28261

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1A (Net1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
GFP-ROCK1

Plasmid

#187296

Purpose

Express GFP ROCK1

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Stargazin-GFP-LOVpep

Plasmid

#80406

Purpose

Membrane localized GFP-tagged LOVpep. Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

Stargazin-GFP-LOVpep (CACNG2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

Plasmid

#173873

Purpose

Mammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLck-mVenus-C1

Plasmid

#84337

Purpose

Expresses a mVenus tagged with a membrane localization sequence

Depositor

Dorus Gadella

Article

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

Lck (MGCVCSSNPELPVAT)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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