Addgene

We narrowed to 11,576 results for: cel.2;

Showing: 161 - 180 of 11576 results

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PX459 mDnd1#2

Plasmid

#106346

Purpose

small guide RNA #2 against RRM-coding region of Dnd1 gene locus

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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px330-Pten-2

Plasmid

#162552

Purpose

sgRNA targeting Pten

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Pten (Pten Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-2

Plasmid

#162549

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMCB320 sgCdkn1a.2

Plasmid

#227946

Purpose

sgRNA targeting Cdkn1a, mCherry, puromycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ (2025)

Insert

Cdkn1a.2 (Cdkn1a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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px330-Kmt2c-2

Plasmid

#162532

Purpose

sgRNA targeting Kmt2c

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Kmt2c (Kmt2c Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Kmt2b-2

Plasmid

#162536

Purpose

sgRNA targeting Kmt2b

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Kmt2b (Kmt2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Alb-2

Plasmid

#162545

Purpose

sgRNA targeting Alb

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Alb (Alb Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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O-TREM2-2

Plasmid

#166545

Purpose

scFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP670-2

Plasmid

#197238

Purpose

Expresses the fusion protein of Tubulin-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pER-eZinCh-2

Plasmid

#70009

Purpose

expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

Depositor

Maarten Merkx

Article

Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

Insert

ER-eZinCh-2

Use

Tags

KDEL sequence and PPI signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Activin A (S2-2)

Plasmid

#184621

Purpose

for transfection into S2 cells and expression of Activin A protein

Depositor

Timothy Springer

Article

Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

Insert

INHBA (INHBA Human)

Use

Tags

8x His - streptavidin binding peptide

Expression

Insect

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMCherry F8-2

Plasmid

#186034

Purpose

Express VHH F8-2 as a mCherry fusion in mammalian cells

Depositor

Isabelle Landrieu

Article

Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

Insert

VHH F8-2

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMMDS 2 colors

Plasmid

#206265

Purpose

Expression of 2 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

GST mTagBFP1 (Synthetic), Ctnnb1 (M. musculus) (Ctnnb1 )

Use

Cre-donor

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir129-2-2

Plasmid

#46728

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir129-2-2 (MIR129-2 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo)

Plasmid

#22131

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo/pTER shEGFP#2 (w22-2)

Plasmid

#17480

Purpose

3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #2

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcdna DSG-2 Tailless

Plasmid

#89466

Purpose

DSG-2 tailless control

Depositor

Daniel Conway

Article

Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

Insert

desmoglein-2 (DSG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcdna DSG-2 TS

Plasmid

#89465

Purpose

DSG-2 tension sensor, using TSmod inserted in human DSG-2

Depositor

Daniel Conway

Article

Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

Insert

desmoglein-2 (DSG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSicoR (EGFP) shPnky-2

Plasmid

#79142

Purpose

Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre

Depositor

Daniel Lim

Article

Ramos et al Cell Stem Cell. 2015 Apr 2;16(4):439-47.

Insert

shPnky-2 (Gm30731 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 for the shRNA and CMV for EGFP

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-ML1N*2(7Q)

Plasmid

#67679

Purpose

Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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