We narrowed to 2,062 results for: gem

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pGEM mouse islet1 [TJ#28]

Plasmid

#16270

Depositor

Thomas Jessell

Insert

islet1 (Isl1 Mouse)

Expression

Bacterial

Available since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_GEM2-Halo-FRB

Plasmid

#197056

Purpose

Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Publication

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer:cAPX

Plasmid

#84736

Purpose

Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

APX1 (APX1 Mustard Weed)
HyPer2 targeted to nucleus

Tags

NLS-SV40

Expression

Plant

Promoter

pFMV/p35S

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGem-sHyper:sAPX

Plasmid

#84738

Purpose

Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

sAPX (SAPX Mustard Weed)
sHyper (HyPer2 targeted to nucleus)

Tags

nuclear localisation signals from the coding sequ…

Expression

Plant

Promoter

pFMV/p35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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RhGC(BE)_pGEM

Plasmid

#85469

Purpose

The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

Depositor

Peter Hegemann

Publication

Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rhodopsin-guanylyl cyclase

Expression

Mammalian

Promoter

T7 Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMT-Mef2c-tPT2A-GFP

Plasmid

#111771

Purpose

Bicistronic construct: Co-express two genes by 2A peptides

Depositor

Li Qian

Publication

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Insert

Mef2c-tPT2A-GFP (Mef2c Mouse)

Use

Cloning intermediate

Tags

GFP

Promoter

T7

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mOtop1_pGEMHE

Plasmid

#114674

Purpose

This construct was used to express mouse Otop1 in Xenopus laevis oocytes

Depositor

Emily Liman

Publication

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 1 (Otop1 Mouse)

Use

For generating the mrna, which can highly express…

Mutation

SNP (rs49325632): Glycine 396 to Alanine

Promoter

T7

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3003 pGEM E6-AP

Plasmid

#8644

Depositor

Peter Howley

Publication

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pGem-nHyPer:sAPX

Plasmid

#84735

Purpose

Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

sAPX (SAPX Mustard Weed)
nHyper (HyPer2 targeted to nucleus)

Tags

nuclear localisation signals from the coding sequ…

Expression

Plant

Promoter

FMV/35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pGEM-HSPA5P

Plasmid

#44150

Purpose

a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

Depositor

Joan Conaway

Publication

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

HSPA5 promoter (HSPA5 Human)

Expression

Mammalian

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-GluCl_cryst

Plasmid

#31487

Depositor

Eric Gouaux

Publication

Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

Insert

C. elegans glutamate-gated chloride channel alpha, crystallized construct

Use

For rna synthesis for expression in x. laevis ooc…

Tags

8x-His

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pGEMHE-mScarlet-hCenpC

Plasmid

#171490

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

Depositor

Melina Schuh

Publication

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

CenpC (CENPC Human)

Use

Pgehme

Tags

mScarlet

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer2:sHyper2

Plasmid

#84737

Purpose

Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Tags

NLS-SV40

Expression

Plant

Promoter

pFMV/p35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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p4020 pGEM HPV-18 E6

Plasmid

#10856

Depositor

Peter Howley

Publication

Werness et al Science. 1990 Apr 6. 248(4951):76-9.

Insert

HPV18 E6 (E6 Human papillomavirus)

Tags

HA?

Expression

Bacterial

Available since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pGEMHE-mCherry-mTrim21ΔRING-Box

Plasmid

#105523

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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UAS-dally

Plasmid

#41175

Depositor

Norbert Perrimon

Publication

Lin et al Nature. 1999 Jul 15;400(6741):281-4.

Insert

dally (dally Fly)

Expression

Insect

Promoter

Hsp70

Available since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
pGEM-2t

Plasmid

#159752

Purpose

CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.

Depositor

Wolfgang Lukowitz

Publication

Angulo et al bioRxiv

Insert

sgRNA

Use

CRISPR

Available since

Oct. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mOtop2_pGEMHE

Plasmid

#114675

Purpose

This construct was used to express mouse Otop2 in Xenopus laevis oocytes

Depositor

Emily Liman

Publication

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Otopetrin 2 (Otop2 Mouse)

Use

For generating the mrna, which can highly express…

Promoter

T7

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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p3523 pGEM HPV-11 E6

Plasmid

#10854

Depositor

Peter Howley

Publication

Werness et al Science. 1990 Apr 6. 248(4951):76-9.

Insert

HPV11 E6

Tags

HA

Expression

Bacterial

Available since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pGEM-hu FceRI beta

Plasmid

#8366

Depositor

Jean Kinet

Publication

Kuster et al J Biol Chem 1992 Jun 25;267(18):12782-7.

Insert

human Fc epsilon RI beta cDNA (MS4A2 Human)

Expression

Bacterial

Available since

June 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article