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We narrowed to 2,726 results for: gem

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Showing: 161 - 180 of 2726 results

  2. CMV-GEM-GECO1

    Plasmid
    #32442
    Purpose
    Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEM-GECO1
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32442-plasmid-map-sequence-id-117095

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  10. pGEMHE-NLS-mEGFP

    Plasmid
    #105527
    Purpose
    T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal
    Depositor
    Melina Schuh
    Article
    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31
    Insert
    Nucleoplasmin
    Use
    In vitro transcription
    Tags
    mEGFP
    Mutation
    NLS of nucleoplasmin (encoding amino acids KRPAAT…
    Promoter
    T7
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    105527-plasmid-map-sequence-id-201844

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  12. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Article
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available Since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80424-plasmid-map-sequence-id-151441

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  13. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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Showing: 161 - 180 of 2726 results