We narrowed to 2,481 results for: gem

1. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMHE-mCherry-mTrim21ΔPRYSPRY

   Plasmid

   #105524

   Purpose

   T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mCherry

   Expression

   Mutation

   deletion of PRYSPRY domain (aa1-281)

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-mClover3-KASH5-DN

   Plasmid

   #171492

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   KASH5-DN (CCDC155 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMHE-GST-KASH5-DN

   Plasmid

   #171493

   Purpose

   T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   KASH5-DN (CCDC155 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   GST

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM cCol2 (CT#617)

   Plasmid

   #13943

   Depositor

   Cliff Tabin

   Article

   Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

   Insert

   Collagen II (COL2A1 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Nucleotides 182-616

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pGEM cAgg (CT#689)

   Plasmid

   #13945

   Depositor

   Cliff Tabin

   Article

   Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

   Insert

   Aggrecan (ACAN Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Nucleotides 113-800

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pCW-GemininΔDbox-HA-Puro

   Plasmid

   #193770

   Purpose

   Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   GemininΔDbox-HA (GMNN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Dbox removed (R23A, L26A). Codons switched at siR…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-mScarlet-hCenpC

   Plasmid

   #171490

   Purpose

   T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   CenpC (CENPC Human)
   

   Use

   Pgehme

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-SNAP-TRIM21

   Plasmid

   #196181

   Purpose

   For in vitro transcription of SNAP-TRIM21 mRNA

   Depositor

   Binyam Mogessie

   Article

   Dunkley et al Sci Adv. 2023 Jan 20;9(3):eadc9161. doi:

   Insert

   SNAP-TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1_GEM22-Halo-FRB

    Plasmid

    #197059

    Purpose

    Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_FRB-GEM23-Halo

    Plasmid

    #197060

    Purpose

    Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    I3-01
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLBS.EF/miRFP670-hGem110Nt

    Plasmid

    #129333

    Purpose

    Cell cycle reporter, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    miRFP670 (Addgene 80006)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEM-T Easy-AtpU6-26

    Plasmid

    #160218

    Purpose

    AtpU6-26 Golden Gate level 0 piece to express gRNAs

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtU6-26 promoter
    

    Use

    Golden gate level 0 piece to express grnas

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGem-nHyPer2:sHyper2

    Plasmid

    #84737

    Purpose

    Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2 targeted to nucleus
    

    Use

    Tags

    NLS-SV40

    Expression

    Plant

    Mutation

    Promoter

    pFMV/p35S

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-Pom121-mScarlet

    Plasmid

    #171491

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEM-H2B-mRFP

    Plasmid

    #196180

    Purpose

    For in vitro transcription of H2B-mRFP mRNA

    Depositor

    Binyam Mogessie

    Article

    Dunkley et al Sci Adv. 2023 Jan 20;9(3):eadc9161. doi:

    Insert

    H2B-mRFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMHE-H2B-mClover3

    Plasmid

    #171489

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged human H2B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H2B (H2BC11 Human)
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2207 pGEM p53

    Plasmid

    #10853

    Depositor

    Peter Howley

    Article

    Werness et al Science. 1990 Apr 6. 248(4951):76-9.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p4020 pGEM HPV-18 E6

    Plasmid

    #10856

    Depositor

    Peter Howley

    Article

    Werness et al Science. 1990 Apr 6. 248(4951):76-9.

    Insert

    HPV18 E6 (E6 Human papillomavirus)
    

    Use

    Tags

    HA?

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pGEM-T Dmel Or9a

    Plasmid

    #59637

    Purpose

    Probe for Drosophila melanogaster Or9a expression

    Depositor

    Richard Axel, Leslie Vosshall

    Article

    Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

    Insert

    Or9a (Or9a Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only