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ND6-L1397-N_Q1310A_right

Plasmid

#187849

Purpose

expresses the right-side half ND6-Q1310A in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-Right-half-120bp

Plasmid

#169205

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-Right-half-120bp-hairpin

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50-0-50%GC-Right-half-120bp

Plasmid

#169207

Purpose

50-0-50%GC-Right-half-120bp-hairpin

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50-0-50%GC-Right-half-120bp-hairpin

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/His-D-iLight

Plasmid

#170270

Purpose

Expression of iLight with 6xHis N-terminal tag in bacteria.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

iLight

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

See Comments

Promoter

araBAD

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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IgH intron enhancer

Plasmid

#127247

Purpose

To maximize gene expression in B cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

heavy chain enhancer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3_RR_NvFP-7R_Ex2-RIGHT

Plasmid

#90037

Purpose

TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

Depositor

Matthew Gibson

Article

Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

Insert

7R_Ex2-RIGHT

Use

TALEN

Tags

Fok1-RR domain and HA

Expression

Mutation

Promoter

CMV

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTGV-Light SMR5452

Plasmid

#28032

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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ET8-GPIba-high-6His

Plasmid

#180251

Purpose

High affinity mutant of human platelet membrane protein GP1b alpha

Depositor

Timothy Springer

Article

Jiang et al J Mol Biol. 2019 Mar 29;431(7):1380-1396

Insert

human GPIbalpha 1-290aa with mutation G233V and M239V (GP1BA Human)

Use

Tags

6His

Expression

Mammalian

Mutation

G233V and M239V

Promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TREtight-mTagBFP2-B19G

Plasmid

#100799

Purpose

helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

Depositor

Ian Wickersham

Article

Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

Has Service

AAV1

Insert

mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TREtight

Available since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 light chain

Plasmid

#175358

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 light chain

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 light chain

Use

Tags

N-terminal secretion signal peptide (MEAPAQLLFLLL…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-pMagHigh-FlpC

Plasmid

#180586

Purpose

PA-Flp

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro-KrasG12D2B

Plasmid

#139786

Purpose

Lentiviral tet-inducible expression of KrasG12D (mouse)

Depositor

Joan Massague

Article

Su et al Nature. 2020 Jan;577(7791):566-571. doi:

Insert

KrasG12D (Kras Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G12D

Promoter

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)

Plasmid

#221997

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: S1330I, A1341V, N1342S, E1370K, T1380I

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3s-ND5-Right-G1397-N

Plasmid

#169213

Purpose

Express DdCBE for ND5 Point Mutation

Depositor

Jin-Soo Kim

Article

Lee et al Nat Commun. 2021 Feb 19;12(1):1190. doi:

Insert

DdCBE for ND5 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-GpNLuc

Plasmid

#70185

Purpose

Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects..

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV)

Plasmid

#140160

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-DIO-SPOTlight-U2GCR

Plasmid

#231889

Purpose

Cre-dependent reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Insert

DIO-SPOTlight-U2GCR

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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