We narrowed to 14,881 results for: pET

1. pET28c-Ku

   Plasmid

   #161936

   Purpose

   Expresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purification

   Depositor

   Harris Wang

   Article

   Yim et al ACS Synth Biol. 2020 Sep 29. doi: 10.102

   Insert

   ku (mku Mycobacterium tuberculosis)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_SUMO_AcCas12n

   Plasmid

   #203808

   Purpose

   pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   SUMO_AcCas12n
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLI1_pet28a

   Plasmid

   #131641

   Purpose

   Bacterial expression of ETS gene FLI1

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   FLI1 (FLI1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET26b_Nb.AD101

   Plasmid

   #131402

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

   Depositor

   Andrew Kruse

   Article

   McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

   Insert

   Nanobody AD101
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET19b+H3

   Plasmid

   #157807

   Purpose

   Expression of cysteine-free human histone H3 (c111A)

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C111A

   Promoter

   T7

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM33_Nsp10

   Plasmid

   #156479

   Purpose

   Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ELK1_pet28a

   Plasmid

   #131647

   Purpose

   Bacterial expression of ETS gene ELK1

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELK1 (ELK1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET15p65

   Plasmid

   #27661

   Depositor

   Kathleen Collins

   Article

   Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):25

   Insert

   Tetrahymena telomerase holoenzyme p65
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pETM33_EIF4E_eiF4E

   Plasmid

   #178474

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   EIF4E_eiF4E (EIF4E Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET23a_mod_HuD_FL

    Plasmid

    #65751

    Purpose

    plasmid for bacterial overexpression of HuD

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    6X His and FLAG HA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM33_Nsp4

    Plasmid

    #156474

    Purpose

    Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM33_USP7_MATH

    Plasmid

    #178490

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    USP7_MATH (USP7 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET29_LuxSit_i

    Plasmid

    #194299

    Purpose

    Bacterial expression of the de novo luciferase, LuxSit-i

    Depositor

    Andy Yeh, David Baker

    Article

    Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

    Insert

    LuxSit-i
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a_PTK2b

    Plasmid

    #224237

    Purpose

    Expression of human PTK2b Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    ptk2b (PTK2B Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a_SYK

    Plasmid

    #224240

    Purpose

    Expression of human SYK Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    syk (SYK Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_LYN

    Plasmid

    #224238

    Purpose

    Expression of human LYN Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    lyn (LYN Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28CLY9

    Plasmid

    #21769

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pET11b_MutT

    Plasmid

    #73155

    Purpose

    Expresses in E. coli, E coli MutT

    Depositor

    Sandra Gabelli, Maurice Bessman

    Article

    Bessman et al J Biol Chem. 1996 Oct 11;271(41):25059-6

    Insert

    MutT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pET11_Ec_ORF209

    Plasmid

    #69029

    Purpose

    Expresses E.coli ORF209/ADPRase in bacterial cells

    Depositor

    Sandra Gabelli, Maurice Bessman

    Article

    Gabelli et al Nat Struct Biol. 2001 May;8(5):467-72.

    Insert

    ORF209/ADPRase
    

    Use

    Tags

    -

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pGreen_GUS_competitor

    Plasmid

    #55208

    Purpose

    GUS-based microRNA target competitor used for testing function as target mimicry

    Depositor

    Michael Axtell

    Article

    Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

    Insert

    beta-glucuronidase
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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