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We narrowed to 325 results for: pet-28

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Showing: 161 - 180 of 325 results

  9. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162666-plasmid-map-sequence-id-321465

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  10. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Ruth Sommese
    Publication
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    BCKDK (BCKDK Human)
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-B…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232116-plasmid-map-sequence-id-464924

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  14. pETcon-pGAL1-Aga2-α-Synuclein-c_myc

    Plasmid
    #225222
    Purpose
    Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.
    Depositor
    Urartu Seker
    Publication
    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360
    Inserts
    α-Synuclein (SNCA Human)
    Aga2 (AGA2 Budding Yeast)
    Tags
    GS linker, HA Tag, and c-myc Tag
    Expression
    Yeast
    Promoter
    GAL1
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    225222-plasmid-map-sequence-id-458386

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  15. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162668-plasmid-map-sequence-id-321523

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  16. pET-His-SUMO-NrdJ-1C(N145A)-SUMO

    Plasmid
    #258594
    Purpose
    Expression of His-SUMO tagged NrdJ-1 C intein with an extra SUMO at C-ternimus in bacteria. Originally as splicing deficient mutant for ITC experiment
    Depositor
    Thomas Muir
    Publication
    Ye et al Chem Sci. 2026 Apr 28. doi: 10.1039/d6sc
    Insert
    His-SUMO-NrdJ-1C(N145A)-SUMO
    Tags
    6xHis-SUMO
    Expression
    Bacterial
    Mutation
    N145A, splicing deficient mutation on C intein
    Available since
    Aug. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    258594-plasmid-map-sequence-id-518777

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  19. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162680-plasmid-map-sequence-id-321455

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  20. pCJ199

    Plasmid
    #162672
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162672-plasmid-map-sequence-id-321466

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Showing: 161 - 180 of 325 results