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Showing: 161 - 180 of 1351 results

  2. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Expression
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87258-plasmid-map-sequence-id-176556

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  4. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87259
    Purpose
    YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-mAID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and mAID
    Expression
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87259-plasmid-map-sequence-id-176555

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  5. pAAV-shCTRL

    Plasmid
    #181875
    Purpose
    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    control shRNA with no known targets in the mouse genome
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181875-plasmid-map-sequence-id-355955

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  6. pTet-On-TAP

    Plasmid
    #59017
    Purpose
    Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Type
    Empty backbone
    Use
    Toxoplasma expression
    Tags
    Calmodulin Binding Peptide fused with a TEV Cleav…
    Expression
    Mutation
    Promoter
    RPS13 with Tet Operators Inserted
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59017-plasmid-map-sequence-id-423220

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  17. pFendoNA2S

    Plasmid
    #174730
    Purpose
    Expresses GFP-endoNA2-S706A fusion protein for detection of polysialic acid. The endoNA2-S706A is a noncatalytic endosialidase that lacks the ability for releasing its C-terminal chaperone domain.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    endoNA2-S706A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed Serine 706 to Alanine
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174730-plasmid-map-sequence-id-343521

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  19. pAAV-4mtD3cpv

    Plasmid
    #181874
    Purpose
    Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    4mtD3cpv
    Use
    AAV and Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    the 4mtD3cpv CDS differs from the recorded GeneBa…
    Promoter
    CaMKIIa
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    181874-plasmid-map-sequence-id-355965

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  20. pAAV-shNCLX-2

    Plasmid
    #181871
    Purpose
    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    solute carrier 8 family member B1 (Slc8b1 Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181871-plasmid-map-sequence-id-355961

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Showing: 161 - 180 of 1351 results