We narrowed to 15,034 results for: pET

1. pET21-pelB-LaG-12

   Plasmid

   #172744

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-12
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-14

   Plasmid

   #172745

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-14
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-11a HIV-1 P6

   Plasmid

   #154201

   Purpose

   Bacterial expression of Gag P6 from HIV-1 HXB2 strain

   Depositor

   Lalit Deshmukh

   Article

   Ramaraju et al Biochemistry. 2021 Aug 3. doi: 10.1021/a

   Insert

   Gag P6 protein from HIV-1 clone HXB2
   

   Use

   Tags

   GB1-6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-DEST42-dre4

   Plasmid

   #170440

   Purpose

   Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

   Depositor

   Joan Conaway

   Article

   Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

   Insert

   dre4 (dre4 Fly)
   

   Use

   Tags

   6xHis and V5

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-20

   Promoter

   T7

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-DEST42 -SSRP1

   Plasmid

   #170439

   Purpose

   Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

   Depositor

   Joan Conaway

   Article

   Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

   Insert

   ssrp1 (Ssrp Fly)
   

   Use

   Tags

   6xHis and V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. human TLNRD1-4H pET151

   Plasmid

   #159386

   Purpose

   Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

   Depositor

   Ben Goult

   Article

   Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
   

   Use

   Tags

   his-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   TLNRD1 4-helix domain (residues 143-273)

   Promoter

   T7

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30 W96A-GLTP

   Plasmid

   #170734

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

   Insert

   W96A-GLTP (GLTP Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   W96A

   Promoter

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a Ec-iPGM-bio

   Plasmid

   #162575

   Purpose

   Expresses E. coli iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   E. coli iPGM bio-6His
   

   Use

   Tags

   6His tag, T7 tag, biotinylation sequence, and thr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-CVm-Sec22b

   Plasmid

   #168029

   Purpose

   Expresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   eCFP-Sec22b(A131-H190)-mVenus
   

   Use

   Tags

   Hisx6 and myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-CVm-VAMP8

    Plasmid

    #168028

    Purpose

    Expresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP8(G7-R67)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28_hTLN2-head-LBD

    Plasmid

    #164837

    Purpose

    Expression of human talin2 head mutant

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN2 (TLN2 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Lipid binding deficient mutations

    Promoter

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET - FnR062.Y78V

    Plasmid

    #84801

    Purpose

    bacterial expression of FnR.0.6.2-Y78V protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78V
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78V

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pET - FnR062.Y78A

    Plasmid

    #84784

    Purpose

    bacterial expression of FnR.0.6.2-Y78A protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78A
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78A

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pET - FnR062.Y78C

    Plasmid

    #84785

    Purpose

    bacterial expression of FnR.0.6.2-Y78C protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78C
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78C

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pET - FnR062.Y78E

    Plasmid

    #84787

    Purpose

    bacterial expression of FnR.0.6.2-Y78E protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78E
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78E

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pET - FnR062.Y78L

    Plasmid

    #84793

    Purpose

    bacterial expression of FnR.0.6.2-Y78L protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78L
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78L

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pET - FnR062.Y78M

    Plasmid

    #84794

    Purpose

    bacterial expression of FnR.0.6.2-Y78M protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78M
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78M

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pET - FnR062.Y78N

    Plasmid

    #84795

    Purpose

    bacterial expression of FnR.0.6.2-Y78N protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78N
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78N

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pET - FnR062.Y78Q

    Plasmid

    #84797

    Purpose

    bacterial expression of FnR.0.6.2-Y78Q protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78Q
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78Q

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pET - FnR062.Y78T

    Plasmid

    #84800

    Purpose

    bacterial expression of FnR.0.6.2-Y78T protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78T

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

21. pET-11a (D3 - L39V)

    Plasmid

    #136607

    Purpose

    Expresses DANCER-3 mutant (reversion L39V)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (L39V reversion)
    

    Use

    Tags

    5x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V54I

    Promoter

    T7 promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-11a (D3 - I54V)

    Plasmid

    #136608

    Purpose

    Expresses DANCER-3 mutant (reversion I54V)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (I54V reversion)
    

    Use

    Tags

    6x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39L

    Promoter

    T7 promoter

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-11a (D3 - F34A)

    Plasmid

    #136606

    Purpose

    Expresses DANCER-3 mutant (reversion F34A)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (F34A reversion)
    

    Use

    Tags

    6x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-V39L-V54I

    Promoter

    T7 promoter

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-11a (D3 - I7L)

    Plasmid

    #136605

    Purpose

    Expresses DANCER-3 mutant (reversion I7L)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (I7L reversion)
    

    Use

    Tags

    6x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-A34F-V39L-V54I

    Promoter

    T7 promoter

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-11a (D3 - F3Y)

    Plasmid

    #136604

    Purpose

    Expresses DANCER-3 mutant (reversion F3Y)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (F3Y reversion)
    

    Use

    Tags

    6x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: L7I-A34F-V39L-V54I

    Promoter

    T7 promoter

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. lvl0 YPet CTAG

    Plasmid

    #153402

    Purpose

    Yellow fluorescent protein CTAG codon optimised for Arabidopsis thaliana

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    Ypet CTAG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET11b-MIF H62F

    Plasmid

    #149445

    Purpose

    Expression of MIF H62F in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H62F

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET11b-MIF H62A

    Plasmid

    #149443

    Purpose

    Expression of MIF H62A in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H62A

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET11b-MIF H62Y

    Plasmid

    #149442

    Purpose

    Expression of MIF H62Y in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H62Y

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET11b-MIF Y99G

    Plasmid

    #149441

    Purpose

    Expression of MIF Y99G in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y99G

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge