We narrowed to 18,759 results for: nls
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Plasmid#234066PurposeEncodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°CDepositorInsertMeltNLS/NES-40-mCherry
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-opCas12a-6xNLS
Plasmid#209022PurposeLentiviral vector expressing human codon-optimized OpCas12a with 6xNLSDepositorInsertopCas12a-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/S542RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVf-EnhCB-lacZnls
Plasmid#35642DepositorTypeEmpty backboneUseAAVAvailable sinceApril 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Grp
Plasmid#184292PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting GrpDepositorInsertsgRNA-Grp
UseAAV and CRISPRExpressionMammalianAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Oprl1
Plasmid#184291PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Oprl1DepositorInsertsgRNA-Oprl1
UseAAV and CRISPRExpressionMammalianAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3
Plasmid#184290PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3DepositorInsertsgRNA-Rxfp3
UseAAV and CRISPRExpressionMammalianAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1
Plasmid#184295PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1DepositorInsertsgRNA-Adrb1
UseAAV and CRISPRExpressionMammalianAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS
Plasmid#145027PurposeLentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear markerDepositorInsert3xnls-Tq-Ca-FLITS
UseLentiviralTags3xnls-Tq-Ca-FLITS and H2B-MaroonExpressionMammalianPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro
Plasmid#136262PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreen-NLSExpressionMammalianAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hSyn ArcLightCo (Q239)-T2A-nls-mCherry
Plasmid#85844PurposeGenetically encoded voltage sensor ArcLight- codon optimizedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArcLightCo-Q239
TagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterhSyn1Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA
Plasmid#108747PurposeExpresses N-terminus to aa27 of Flp recombinase fused to GID1 CIDDepositorInsertFlp recombinase fragment N-terminus to aa27
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Col1A2:TFPnls-T2a-mErt-Cre-Ert
Plasmid#111151PurposeFibroblasts specific inducible CreDepositorInsertTFPnls-T2a-mErt-Cre-Ert
UseCre/LoxMutationwtPromoterCol1A2Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFUW-TetO-mCherry-cNLS
Plasmid#138531PurposeLentiviral inducible expression of mCherry with nuclear localization signalDepositorInsertmCherry
UseLentiviralTags3x SV40 cNLSExpressionMammalianPromoterTRE-CMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFUW-TetO-tdTomato-cNLS
Plasmid#138532PurposeLentiviral inducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
UseLentiviralTags3x SV40 cNLSExpressionMammalianPromoterTRE-CMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMZ-180
Plasmid#195428PurposeTransient, constitutive expression of synZiFTR: ZF5-p65DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpUb - 3XFLAG - NLS - ZF5-7 (ZF5) - p65 - RbGpA
ExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD0257 pCS2-sfCherry11Mx4-10aa-zH2B-SV40NLS-sfGFP-NPLnls
Plasmid#251772PurposeNuclear reporter for fluorescent protein reconstitution assayDepositorPublicationInsertH2B (hist2h2l Zebrafish)
Tags4x sfCherry3C small fragments, NLS (SV40) and NLS…ExpressionMammalianPromoterCMV IE94Available sinceApril 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101103PurposeEncodes dCas9(C)-VP64 with SAM components fused to VEGFR1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVEGFR1-dCas9(C)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Prrx1:TFPnls-T2a-Cre-Ert
Plasmid#111150PurposeLimb connective tissue specific inducible CreDepositorInsertTFPnls-T2a-Cre-Ert
UseCre/LoxMutationwtAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-His6-FnCas9GFP
Plasmid#130966Purposeexpression of FnCas9-GFP in bacterial cellsDepositorInsertHA-NLS-FnCas9
UseCRISPRTags6xHis-mEGFP-TEV-HA-NLSExpressionBacterialPromoterT7Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiCRISPRv1-sgPHGDH-G5
Plasmid#83913Purposestable knockoutDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG
Plasmid#225245PurposeMammalian expression of iPASnFR-H in the nucleusDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiPASnFR-H
Tags3xFLAG and mScarlet3ExpressionMammalianAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)
Plasmid#101105PurposeEncodes dCas9(C) (without VP64) fused to VEGFR1DepositorInsertVEGFR1-dCas9(C)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-nlsABACUS2-400n
Plasmid#203726PurposeGateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnlsABACUS2-400n
UseSynthetic BiologyTagsMycAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-enCas12a-2xNLS
Plasmid#209021PurposeLentiviral vector expressing human codon-optimized enCas12a with 2xNLSDepositorInsertenCas12a-2xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationE174R/S542R/K548RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS-nlstdTomato
Plasmid#67693PurposeTol1 expression plasmid with UAS-driven myc tagged nlsTdTomatoDepositorInsert6xMTnlsTomato
Tags6xMyc-NLSAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
mVenus-PCNA-19-SV40NLS-4
Plasmid#56621PurposeLocalization: Nucleus, Excitation: 515, Emission: 528DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCNA-19 (PCNA Human)
Tags3xSV40NLS and mVenusExpressionMammalianPromoterCMVAvailable sinceDec. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA
Plasmid#220224PurposeAAV-STARR-seq validation control vector with VenusDepositorTypeEmpty backboneUseAAVExpressionMammalianPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM36
Plasmid#261123PurposebFOSdeltaZip::VC155 and mCherry expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertrgef-1p::AID::nls::mcherry::2a::AID::bFOSdeltaZIP::VC155::tbb-2 3' in pLF3FShC
ExpressionWormMutationbFOSdeltaZIPPromoterrgef-1Available sinceOct. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCM40
Plasmid#261127PurposebFOS::VC155 and mCherry expression under myo-3 promoter expression. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertmyo-3p::AID::nls::mcherry::2a::AID::bFOS::VC155::tbb-2 3' in pLF3FShC
ExpressionWormMutationbFOSPromotermyo-3Available sinceSept. 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCM42
Plasmid#261129PurposebJJUN::VN173 and mTagBFP expression under myo-3 promoter expression. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertmyo-3p::AID::nls::mTagbfp2::2a::AID::bJUN::VN173::tbb-2 3' in pLF3FShC
ExpressionWormMutationbJUNPromotermyo-3Available sinceSept. 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCM41
Plasmid#261128PurposebFOSdeltaZip::VC155 and mCherry expression under myo-3 promoter expression. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertmyo-3p::AID::nls::mcherry::2a::AID::bFOSdeltaZIP::VC155::tbb-2 3' in pLF3FShC
ExpressionWormMutationbFOSdeltaZIPPromotermyo-3Available sinceSept. 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCM37
Plasmid#261124PurposebJUN::VN173 and mTagBFP expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertrgef-1p::AID::nls::mTagbfp2::2a::AID::bJUN::VN173::tbb-2 3' in pLF3FShC
ExpressionWormMutationbJUNPromotermyo-3Available sinceAug. 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCM35
Plasmid#261122PurposebFOS::VC155 and mCherry expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertrgef-1p::AID::nls::mcherry::2a::AID::bFOS::VC155::tbb-2 3'in pLF3FShC
ExpressionWormMutationbFOSPromoterrgef-1Available sinceAug. 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
L2_2xNOP1gRNA-Cas9-CsA
Plasmid#136140PurposePlasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygRDepositorInsertp5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM266 - pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Plasmid#159829PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertpEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Plasmid#159833PurposeSingle copy or array insertion by MosTI using split NeoR selectionDepositorInsertpEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE
Plasmid#138143PurposeExpresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caacDepositorInsertPguCas13b
UseLentiviralTagsNLSAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLEX.(NLS)iGlucoSnFR.H66A.L276V
Plasmid#238944PurposeGreen glucose sensor, high sensitivity, nuclearDepositorInsertiGlucoSnFR.L276V.K312A
UseLentiviralTagsMyc and NLSExpressionMammalianPromoterCMVAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBP60g-NLS-3xmCitrine
Plasmid#192521PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertCALMODULIN BINDING PROTEIN 60g
Tags3xmCitrine and NLSExpressionPlantPromoterCBP60gAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only