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We narrowed to 15,323 results for: pET

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Showing: 4451 - 4500 of 15323 results

  1. pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184741
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184741-plasmid-map-sequence-id-361527

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  2. pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI

    Plasmid
    #184742
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 1 transcribed by BBa_J23103
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184742-plasmid-map-sequence-id-361529

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  3. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184739
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184739-plasmid-map-sequence-id-361530

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  4. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184740
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184740-plasmid-map-sequence-id-361528

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  5. pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

    Plasmid
    #117005
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    117005-plasmid-map-sequence-id-227039

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  6. pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184738
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184738-plasmid-map-sequence-id-361531

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  8. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid
    #193253
    Purpose
    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Depositor
    Mark Sellmyer
    Article
    Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1
    Insert
    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Use
    Lentiviral
    Tags
    Renilla luciferase (rLuc) and yellow fluorescent …
    Mutation
    None
    Promoter
    EF1a
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    193253-plasmid-map-sequence-id-383064

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  9. pT7-pelB

    Plasmid
    #157740
    Purpose
    empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    PelB signal sequence and possible His6-tag (adds …
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157740-plasmid-map-sequence-id-334002

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  19. pHIT198

    Plasmid
    #204509
    Purpose
    pET-derived vector for expression of proteins N-terminally tagged with 6xHis and maltose binding protein (multi-cloning sites: NdeI, NotI, SpeI, SalI, PstI, SgfI, and XhoI)
    Depositor
    Yuji Kohara
    Article
    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.
    Type
    Empty backbone
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204509-plasmid-map-sequence-id-403818

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  23. pBP-Tag_linker

    Plasmid
    #72982
    Purpose
    N-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    N-terminal linker with pET-RBS
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72982-plasmid-map-sequence-id-133829

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  24. pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201820
    Purpose
    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging
    Depositor
    Christopher Richie
    Article
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Promoter
    SYN1
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201820-plasmid-map-sequence-id-399615

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  25. pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201821
    Purpose
    An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET
    Depositor
    Christopher Richie
    Article
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Promoter
    SYN1
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201821-plasmid-map-sequence-id-399618

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  26. pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201822
    Purpose
    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging
    Depositor
    Christopher Richie
    Article
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Promoter
    CaMKii
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201822-plasmid-map-sequence-id-399617

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  40. pCIOX

    Plasmid
    #51300
    Purpose
    E.coli expression vector for His8-SUMO tagged recombinant protein
    Depositor
    Andrea Mattevi
    Type
    Empty backbone
    Tags
    His8 + SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    51300-plasmid-map-sequence-id-78111

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  49. pSG059

    Plasmid
    #214262
    Purpose
    PaFtsH2-6xHis cloned in XbaI/NheI site in pET-28a(+)
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    tLST accessory element PaFtsH2 protease of Pseudomonas aeruginosa SG17M
    Tags
    6x His-tag and 6xHis-tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214262-plasmid-map-sequence-id-432470

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Showing: 4451 - 4500 of 15323 results