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pAdEasy-Msln-iCre-HA-Flag

Plasmid

#31305

Depositor

William Pu

Article

Zhou et al J Clin Invest. 2011 May 2. 121(5):1894-9

Insert

Mesothelin (MSLN Human)

Use

Adenoviral

Tags

Flag and HA

Expression

Mutation

Promoter

Available since

March 1, 2012

Availability

Academic Institutions and Nonprofits only
Lenti_CRISPRi_sgAR2

Plasmid

#167001

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 2 (AR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCSII-EF/mt-(n1)StayGold

Plasmid

#185823

Purpose

A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

Depositor

Atsushi Miyawaki

Article

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

mt-(n1)StayGold

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHDM-Hgpm2

Plasmid

#164441

Purpose

Encodes codon-optimized HIV gagpol for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

Codon Optimized HIV-1 GagPol

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PfV-Sapphire-IRES-DSRed

Plasmid

#116934

Purpose

Mammalian lenti vector for expression of the 40nm GEM and DSRed

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Lentiviral

Tags

Sapphire

Expression

Mutation

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK3-flag

Plasmid

#15496

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK3 (DKK3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

See note below

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-pCMV-PfV-GS-Sapphire

Plasmid

#116933

Purpose

Mammalian vector for expression of the 40nm GEM

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Tags

Sapphire

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAV106-pTDH3-mCherry-10xSumo-6xSIM

Plasmid

#116928

Purpose

Yeast integrative vector for expression of the SUMO-SIM complex

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10xSUMO-6xSIM

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Luc2-IRES-ZsGreen-W

Plasmid

#164432

Purpose

Lentiviral vector backbone encoding firefly luciferase and ZsGreen

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Inserts

Luciferase
ZsGreen

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRC-CMV-Rev1b

Plasmid

#164443

Purpose

Encodes HIV-1 Rev accessory protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

HIV-1 Rev

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3/er-(n2)oxStayGold(c4)

Plasmid

#185822

Purpose

A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

Depositor

Atsushi Miyawaki

Article

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

er-(n2)oxStayGold(c4)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRS306-PHIS3-GFP-μNS

Plasmid

#116935

Purpose

Yeast integrative vector for the expression of μNS

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

μNS

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

HIS3

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK1-flag

Plasmid

#15494

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK1 (DKK1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pmPB2-VSV

Plasmid

#68038

Purpose

Expresses mouse Plexin-B2 with N-terminal VSV tag in mammalian cells

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B2 (Plxnb2 Mouse)

Use

Tags

VSV

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pDONRG_P4-P1R:MUM4_0.3Pro_35S

Plasmid

#128558

Purpose

Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307 bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal promoter sequence, for use in Three-way Gateway cloning

Depositor

George Haughn

Article

Dean et al Plant Mol Biol. 2017 Sep;95(1-2):33-50.

Type

Empty backbone

Use

Gateway promoter entry clone

Tags

Expression

Mutation

Promoter

MUM4 core promoter with 35S enhancer

Available since

Sept. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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HA-cNM2A-Flag

Plasmid

#236532

Purpose

For expression of NM2A (dog) - with HA and FLAG tags in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

MYH9

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HA-mNM2A

Plasmid

#236531

Purpose

For expression of NM2A (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

MYH9 (Myh9 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hGRN2

Plasmid

#213683

Purpose

Expresses human granulin-2 with an N-terminal twin-Strep-FLAG tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

human Granulin-2

Use

AAV

Tags

twin-Strep-FLAG

Expression

Mutation

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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CS2-Myc-dLIS1

Plasmid

#47047

Purpose

Expression of tagged Drosophila LIS1

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Lis1 (Lis-1 Fly)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
CS2-CHY-dLIS1

Plasmid

#47045

Purpose

Expression of fluorescent Drosophila LIS1

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Lis1 (Lis-1 Fly)

Use

Tags

CHY

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only