We narrowed to 5,258 results for: pcDNA3.1+

1. pcDNA3.1(+)Gαi2-91-NLuc

   Plasmid

   #207149

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAI2 (GNAI2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)Q

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-myc-AP1S3

   Plasmid

   #58292

   Purpose

   mammalian expression of AP1S3

   Depositor

   Francesca Capon

   Article

   Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

   Insert

   AP1S3 (AP1S3 Human)
   

   Use

   Tags

   6xHis and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1(+)-Mito-CaNARi

   Plasmid

   #64851

   Purpose

   Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, N-terminal 30 amino acids from DAKAP1, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_PA-Cre-Y324F

   Plasmid

   #122961

   Purpose

   Catalytically inactive mutant of PA-Cre

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

   Insert

   pcDNA3.1_PA-Cre-Y324F
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-MS2-HA-HsMex3C_AD

   Plasmid

   #148481

   Purpose

   Mammalian Expression of HsMex3C

   Depositor

   Elisa Izaurralde

   Insert

   HsMex3C (MEX3C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L11P in Mex3C

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-MS2-HA-HshnRNPH1_Z

   Plasmid

   #148120

   Purpose

   Mammalian Expression of HshnRNPH1

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPH1 (HNRNPH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 (-) hYAP 1-1delta

   Plasmid

   #124142

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Megan Finch-Edmondson, Marius Sudol

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   None (Wild Type)

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 FLIPE-100u

   Plasmid

   #13543

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E S118W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   S118W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(−) mCER-Prx2

   Plasmid

   #158224

   Purpose

   Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

   Depositor

   Tobias Dick

   Article

   Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

   Insert

   mCER-Prx2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CFP-KRAS4B

    Plasmid

    #112717

    Purpose

    Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    CFP-KRAS4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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