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pI1-MTU-DO-LEU

Plasmid

#160013

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pYes2-TaPCS1

Plasmid

#49767

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Article

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

TaPCS1 (PCS1 Triticum aestivum)

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pDRF1-GW mNeongreen-T2A-mCherry

Plasmid

#125698

Purpose

Produces equimolar expression of mNeongreen and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mNeongreen-T2A-mCherry

Expression

Yeast

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHES875

Plasmid

#89192

Purpose

Expresses mCherry under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-mCherry

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-msGFPx3

Plasmid

#105258

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Gga2 portion (GGA2 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinA GFP

Plasmid

#32119

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP and Kar2

Expression

Yeast

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
NM R(delta) 2-5 GFP

Plasmid

#1209

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 NM domain (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

last four repeats of sup35 N domain deleted

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pDR-GW FLIPglu-30μΔ13V

Plasmid

#27998

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-30μΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

D154A in mglB

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
cyterm-ymtq2 pDRF1-GW

Plasmid

#168083

Purpose

Expresses Cyterm-ymTq2 to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymTq2

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHES820

Plasmid

#89190

Purpose

Expresses YFP under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-YFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mScarletI-T2A-mTurquoise2

Plasmid

#118430

Purpose

Produces equimolar expression of mScarletI and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mScarletI-T2A-mTurquoise2

Expression

Yeast

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL2-iGFPx6

Plasmid

#105251

Purpose

Plasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

APL2 portion (APL2 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDR-GW FLIPglu-2µΔ13V

Plasmid

#28000

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-2µΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

H152A

Available Since

June 10, 2011

Availability

Academic Institutions and Nonprofits only
YIplac211-APL5-mCherry2Bx6

Plasmid

#105253

Purpose

Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl5 (APL5 Budding Yeast)

Tags

mCherry2Bx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pNXRVj-HACre

Plasmid

#78407

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
aur1R (aur1 Fission Yeast)

Expression

Yeast

Mutation

Gly 240 to Cys

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-TLG2

Plasmid

#105262

Purpose

Plasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg2 (TLG2 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-ARF1-msGFP

Plasmid

#105255

Purpose

Plasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Arf1 (ARF1 Budding Yeast)

Tags

msGFP

Expression

Yeast

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHES834

Plasmid

#87947

Purpose

Expresses mKate2 under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-mKate2

Use

Synthetic Biology

Expression

Yeast

Promoter

pGal1

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHES854

Plasmid

#89197

Purpose

Expresses mKate2 under control of TDH3 constitutive promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pTDH3-mKate2

Expression

Yeast

Promoter

TDH3

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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NAT-TET-OFF -MET - 3xHA

Plasmid

#182526

Purpose

Doxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

7 Copies of TetO Box

Tags

3HA

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ673

Plasmid

#132953

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Promoter

adh1

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMLB10

Plasmid

#177273

Purpose

GAL1p:T7RNAP(F11L;T613A):CYC1t

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

T7RNAP(F11L;T613A)

Expression

Bacterial and Yeast

Mutation

F11L T613A

Promoter

GAL1

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mKate2-T2A-mTurquoise2

Plasmid

#118426

Purpose

Produces equimolar expression of mKate2 and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mKate2-T2A-mTq2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8

Plasmid

#99354

Purpose

Bacterial expression of fission yeast (S. pombe) Tropomyosin, cdc8

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSEYC68-HA-TOR2 (pAS21)

Plasmid

#39207

Depositor

Michael Hall

Article

Kunz et al J Biol Chem. 2000 Nov 24;275(47):37011-2

Insert

TOR2 (TOR2 Budding Yeast)

Tags

HA

Expression

Yeast

Promoter

GAL

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
cyterm-ymScarletI pDRF1-GW

Plasmid

#168082

Purpose

Expresses Cyterm-ymScarletI to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymScarletI

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW eGFP-T2A-mCherry

Plasmid

#118422

Purpose

Produces equimolar expression of eGFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

eGFP-T2A-mCherry

Expression

Yeast

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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C-SWAT GFP CL1

Plasmid

#182512

Purpose

C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CL1 Degron

Tags

GFP

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL6-msGFPx3

Plasmid

#105254

Purpose

Plasmid for pop-in/pop-out replacement of Apl6 with Apl6-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl6 portion (APL6 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-YPT31

Plasmid

#105263

Purpose

Plasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Ypt31 (YPT31 )

Tags

iGFP

Expression

Yeast

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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