We narrowed to 18,831 results for: Tec

1. pMM0623

   Plasmid

   #128998

   Purpose

   pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM0624

   Plasmid

   #128999

   Purpose

   pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMM0621

   Plasmid

   #128996

   Purpose

   pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330A_FokI-1x7

   Plasmid

   #63607

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-MBP-huCMtCas12a

   Plasmid

   #174680

   Purpose

   Expresses 6xHis-MBP tagged CMtCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huCMtCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCY 3010-07

   Plasmid

   #36212

   Depositor

   Anne Robinson

   Article

   Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

   Insert

   Streptoalloteichus hindustanus bleomycin (Sh ble)
   

   Use

   Yeast cassette for pcr and homologous recombinati…

   Tags

   yEpolylinker-Cerulean-Zeocin

   Expression

   Mutation

   Promoter

   Available Since

   June 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TAL3126

   Plasmid

   #41300

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-nphs2-Left (nphs2 Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pUPD-G418R (01-21)

   Plasmid

   #101703

   Purpose

   G418R in domestication vector

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Insert

   GGAG-[G418R (loxP-PAgTEF-kanMX-TAgTEF-loxP)]-CGCT
   

   Use

   Yeast expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GGE094

   Plasmid

   #120787

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_Zeta-SfiI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GGE091

    Plasmid

    #120786

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_Zeta-SfiI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCfB2400

    Plasmid

    #67552

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. TAL2453

    Plasmid

    #36842

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    RUNX1-TALEN_Right (Runx1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pDTN3997

    Plasmid

    #113337

    Purpose

    Multi-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolytica

    Depositor

    Suzanne Sandmeyer

    Article

    Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

    Insert

    multi-copy 2-pyrone synthase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

14. V179 pLP Triple-Flag - Acceptor

    Plasmid

    #11706

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Acceptor vector

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 26, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pMBD-Gate1

    Plasmid

    #25946

    Depositor

    Kamil Onder

    Article

    Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

    Insert

    
    

    Use

    Tags

    GAL4 Fusion Protein

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSET FLIPE-100u

    Plasmid

    #13539

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    S118W in binding protein

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ291_WT_low

    Plasmid

    #128855

    Purpose

    Low copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SEPT11 sh1 3m shRNA

    Plasmid

    #122021

    Purpose

    Negative control shRNA for SEPT11 sh1 shRNA (plasmid 122020).

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 shRNA (Sept11 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMAD-Gate1

    Plasmid

    #25916

    Depositor

    Kamil Onder

    Article

    Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

    Insert

    
    

    Use

    Tags

    VP16 Fusion Protein

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUPD-Su9MTS (12)

    Plasmid

    #101691

    Purpose

    Su9MTS in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    CCAT-[MTS from Neurospora crassa ATP synthase subunit 9 (OR74A)]-AATG
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMM0617

    Plasmid

    #128993

    Purpose

    loxP-KIURA3-loxP preassembled entry vector

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUCBB-lacZa

    Plasmid

    #32563

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    LacZ-alpha
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available Since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCellFree_G03 TCF25

    Plasmid

    #67114

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCF25 (TCF25 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pCfB6581

    Plasmid

    #106137

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfB6580

    Plasmid

    #106136

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCfB6575

    Plasmid

    #106133

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mEos2-Zyxin-6

    Plasmid

    #57441

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Zyxin (ZYX Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

28. V181 pLPS 3' Triple-Flag - Acceptor

    Plasmid

    #11708

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Acceptor vector

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 26, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. V1579 pLP ProHis HTb - Acceptor

    Plasmid

    #11718

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Acceptor vector

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pCellFree_G03 TFEB

    Plasmid

    #67097

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFEB (TFEB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

31. GGE254

    Plasmid

    #120789

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown_Lip-NotI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GGE255

    Plasmid

    #120788

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_Lip-NotI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. tdEos-FAK-5

    Plasmid

    #57612

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    FAK
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

34. pCAG-TetR-RNF169UBD

    Plasmid

    #119011

    Purpose

    Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    TetR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBLIC-neo

    Plasmid

    #45198

    Purpose

    a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Neo selection.

    Depositor

    Priyamvada Rai

    Article

    Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

36. YIp204-P30-His-POL30

    Plasmid

    #99550

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSox2-bd_mFGF_eGFP

    Plasmid

    #34704

    Depositor

    Hollis Cline

    Article

    Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

    Insert

    eGFP
    

    Use

    Xenopus expression

    Tags

    165-bp minimal FGF4 promoter and 6x Sox2/Oct3-4 t…

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLNL1134

    Plasmid

    #160865

    Purpose

    PJ23110-RBS-ScEHT1-mRuby2-tL3S2P24_KanR_p15A

    Depositor

    John Dueber

    Article

    Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

    Insert

    Medium-chain fatty acid ethyl ester synthase
    

    Use

    Synthetic Biology

    Tags

    mRuby2

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23110

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TAL2343

    Plasmid

    #36763

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    PTCH1-TALEN_Right (PTCH1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pJ211_rha_WT_med

    Plasmid

    #128852

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge