We narrowed to 62,913 results for: cloning

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pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

Plasmid

#110854

Purpose

Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

sgRNA scaffold with spacer

Use

Lentiviral

Mutation

WT

Promoter

U6

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pGL3-U6-sgRNA-EGFP

Plasmid

#107721

Purpose

For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

Depositor

Xingxu Huang

Publication

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pH-nCas9-PBE

Plasmid

#98163

Purpose

Expresses CRISPR base editor in rice

Depositor

Caixia Gao

Publication

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-nCas9-UGI-NLS

Use

Rice

Expression

Plant

Promoter

Ubi

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti6.2-ccdB-Nanoluc

Plasmid

#87075

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Use

Lentiviral

Tags

Nanoluc luciferase

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.2 TDP-43 NLS1 YFP

Plasmid

#84912

Purpose

Mammalian expresion of TDP-43 NLS1 YFP

Depositor

Aaron Gitler

Publication

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

K82A, R83A, K84A

Promoter

CMV

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDEST-hisMBP-AsCpf1-EC

Plasmid

#79007

Purpose

Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Publication

Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

AsCpf1

Tags

6xHis-MBP-NLS and NLS-HA

Expression

Bacterial

Promoter

tac promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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TOPO mKate2

Plasmid

#68441

Purpose

TOPO construct expressing mKate2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKate2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Mutation

R175H

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAVS1-SA-2A-NEO-CAG-RTTA3

Plasmid

#60431

Purpose

neomycin resistance; constitutive promoter driving reverse tet activator

Depositor

Paul Gadue

Publication

Sim et al Methods Mol Biol. 2015 Jan 29.

Insert

rtTA3

Use

Zfn donor

Promoter

CAG

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pENTR1A-Huwe1 C4341S

Plasmid

#37432

Depositor

Jean Cook

Insert

Huwe1 (HUWE1 Human)

Use

Gateway Cloning

Mutation

C4341S

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSCV-N EBNA1

Plasmid

#37954

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

EBNA1 (EBNA-1 H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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p6599 MSCV-IP N-HAonly FOSL1

Plasmid

#34897

Depositor

Peter Howley

Publication

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

FOSL1 (FOSL1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJT305 Pa01 Lenti pEF-attB LSR GFP

Plasmid

#184948

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Publication

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Mm.cargo(Cas9)

Plasmid

#174865

Purpose

Expresses mouse Peg10 cargoRNA encoding SpCas9

Depositor

Feng Zhang

Publication

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

SpCas9 flanked by MmPeg10 UTRs

Tags

HA-SV40 NLS and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCold CL7-Cas9

Plasmid

#124890

Purpose

obtaining full Cas9 RNP

Depositor

Yi Liu

Publication

Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

Insert

Cas9

Expression

Bacterial

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSm-Nuclease

Plasmid

#132431

Purpose

Expresses Serratia marcescens nuclease in E. coli using T7 promoter

Depositor

Mark Arbing

Insert

Serratia marcescens nuclease (codon optimized)

Tags

None

Expression

Bacterial

Promoter

T7

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSGKP-km

Plasmid

#117233

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Publication

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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LRCherry2.1

Plasmid

#108099

Purpose

Lentiviral expression plasmid of sgRNA with mCherry

Depositor

Christopher Vakoc

Publication

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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AAV-CMVc-Cas9

Plasmid

#106431

Purpose

Expresses SpCas9 from the CMVc promoter in AAV backbone

Depositor

Juan Belmonte

Publication

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Cas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMVc

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pT3-EF1aH N90-beta-catenin

Plasmid

#86499

Purpose

Mammalian expression of Myc tagged N90 beta-catenin

Depositor

Xin Chen

Publication

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of amino acids 1-90; constitutively acti…

Promoter

EF1a

Available since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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