We narrowed to 9,088 results for: ef1;

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pU6-mir144 EF1Alpha-puro-T2A-BFP

Plasmid

#164791

Purpose

Express miR-144 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144 (MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

Plasmid

#164800

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA1
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75232

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75236

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA

Plasmid

#37089

Purpose

Expresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-TdTomato

Use

AAV and Cre/Lox; Cre-off

Tags

TdTomato

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

Plasmid

#166413

Purpose

express hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

Protection Of Telomeres 1 (POT1 Human)

Use

Lentiviral

Tags

myc

Expression

Mammalian

Mutation

ΔOB deleted 125 a.a

Promoter

ef1-a

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA1-lenti

Plasmid

#115662

Purpose

Expresses AcrVA1 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

Plasmid

#129704

Purpose

Express HA-KASH for nuclei labeling and purification

Depositor

Yi Zhang

Article

Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

Insert

HA-VHH-KASH

Use

AAV and Cre/Lox

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75237

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pOTTC710 - pAAV EF1a DIO Mem-AcGFP

Plasmid

#75081

Purpose

An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Mem-AcGFP

Use

AAV and Cre/Lox

Tags

Palmitylation site

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE-SOX17-P2A-Venus-rpEF1a-Zeo

Plasmid

#169174

Purpose

piggyBac transposon to generate a Tet inducible SOX17 expressing cell line. Tet-On inducible when paired with #169175

Depositor

Igor Slukvin

Article

Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

Insert

SOX17-P2A-Venus (SOX17 Synthetic, Human)

Use

Piggybac transposon

Expression

Mammalian

Promoter

pTREtight (tet-inducible promoter)

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

Plasmid

#170254

Purpose

Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Mutation

A662C, N664C, V667L

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA

Plasmid

#37088

Purpose

Expresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibition

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

NpHR3.0-EYFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Archon1-KGC-GFP-ER2

Plasmid

#115890

Purpose

AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1a（1.1kb short version)

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

Plasmid

#71219

Purpose

dCas9 fused to the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Use

Lentiviral

Tags

dSpCas9-DNMT3B(E697A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-mouse SmyD1-T2A-copGFP

Plasmid

#101157

Purpose

Lentiviral expression of SmyD1 and copGFP

Depositor

Martine Roussel

Article

Wei et al Oncotarget. 2017 Jul 27. doi: 10.18632/o

Insert

SmyD1 (Smyd1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Egr3-EYFP-WPRE

Plasmid

#186417

Purpose

Overexpression of Egr3 transcript variant 1 CDS under EF-1α promoter

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

Early growth response 3 (Egr3 Mouse)

Use

AAV

Tags

EYFP

Promoter

EF-1-alpha

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)

Plasmid

#100061

Purpose

AAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoter

Depositor

Karl Deisseroth

Has Service

AAV9

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

EF1a

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NES-tdTomato-LINuS-WPRE

Plasmid

#159894

Purpose

AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

Depositor

Tatsushi Onaka

Article

Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

Insert

NES-tdTomato-LINuS

Use

AAV

Expression

Mammalian

Promoter

EF1α promoter

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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