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pCMV/SB11

Plasmid

#26552

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Mutation

Methionine 243 to Glutamine Threonine 136 to Arg…

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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mCherry-Fis1

Plasmid

#182580

Purpose

Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

mCherry-Fis1

Tags

Fis1

Expression

Mammalian

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1

Plasmid

#22027

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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TFORF3119

Plasmid

#144595

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

DDIT3 (DDIT3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV/SB10

Plasmid

#24551

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pIS1_Opt_Renilla

Plasmid

#210571

Purpose

Expresses Optimal Renilla luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla

Use

Luciferase

Expression

Mammalian

Promoter

HSV TK; T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE23

Plasmid

#90365

Purpose

ATF4 - Amino acid deprivation gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

AARE

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE8.8m-WT-P2A-EGFP (BKS953)

Plasmid

#242651

Purpose

CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpCas9(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-ABE8.8m-SpCas9-P2A-EGFP

Use

CRISPR

Expression

Mammalian

Mutation

ABE8.8 mutations in TadA

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SIRT4 Flag

Plasmid

#13815

Purpose

Mammalian expression of human SIRT4 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT4 (SIRT4 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-mVenus-TOSI

Plasmid

#172491

Purpose

mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

Depositor

David Scadden

Article

Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

Insert

Pdcd4 (Pdcd4 Mouse)

Use

Retroviral

Tags

mVenus

Expression

Mammalian

Promoter

MLV LTR

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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