We narrowed to 2,365 results for: DAP

1. pPB-CAG-ARID1A-CBR-FLAG

   Plasmid

   #242212

   Purpose

   Expression of core binding region (CBR) of human ARID1A using piggyBac transposition. ∆IDR1, ∆ARID, and ∆IDR2 (deletion of a.a 2-1610)

   Depositor

   Courtney Hodges

   Article

   Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

   Insert

   ARID1A (ARID1A Human)
   

   Use

   Piggybac

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Core binding region (CBR) only of ARID1A. Deleted…

   Promoter

   CAG

   Available Since

   Sept. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-CAG-ARID1A-deltaARIDIDR2-FLAG

   Plasmid

   #242214

   Purpose

   Expression of human ARID1A ∆ARID and ∆IDR2 (deletion of a.a. 971-1610) using piggyBac transposition

   Depositor

   Courtney Hodges

   Article

   Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

   Insert

   ARID1A (ARID1A Human)
   

   Use

   Piggybac

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deleted ARID and IDR2 from ARID1A (deletion of a.…

   Promoter

   CAG

   Available Since

   Sept. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-CAG-ARID1A-deltaIDR1IDR2-FLAG

   Plasmid

   #242213

   Purpose

   Expression of human ARID1A ∆IDR1 and ∆IDR2 (deletion of a.a. 2-970 and a.a. 1170-1610) using piggyBac transposition

   Depositor

   Courtney Hodges

   Article

   Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

   Insert

   ARID1A (ARID1A Human)
   

   Use

   Piggybac

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deleted IDR1 and IDR2 from ARID1A (deletion of a.…

   Promoter

   CAG

   Available Since

   Sept. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBIG1a_StrepII-Hook3_FTS_FHIP1B

   Plasmid

   #222299

   Purpose

   Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3 (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

   Plasmid

   #222300

   Purpose

   Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3[451-718] (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B

   Plasmid

   #222301

   Purpose

   Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3[571-718] (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHD-dsRed-attP_dCrk_flankingHRs

   Plasmid

   #131141

   Purpose

   Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Inserts

   Crk Upstream Homology Region (Crk Fly)
   Crk Downstream Homology Region (Crk Fly)
   

   Use

   CRISPR and Cre/Lox; Homology directed repair temp…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Martin S. Taylor, Oliver Weichenrieder

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / Lac

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

   Plasmid

   #213031

   Purpose

   Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

   Depositor

   Martin S. Taylor, Donna Romero

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
   

   Use

   Luciferase

   Tags

   Firefly luciferase antisense intron (3' UTR)

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE bidirectional tet-on

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid

    #213030

    Purpose

    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

    Plasmid

    #213025

    Purpose

    Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (1-1275)
    

    Use

    Tags

    3C-3xFlag (ORF2p)

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBP-P_phlF-PPhlF

    Plasmid

    #204075

    Purpose

    Type 0 promoter part

    Depositor

    Mikhail Shapiro

    Insert

    phlF repressor and PPhlF DAPG-inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBP-P_TALEsp1_PUPsp1-phlF_PPhlF

    Plasmid

    #204080

    Purpose

    Type 0 promoter part

    Depositor

    Mikhail Shapiro

    Insert

    TALEsp1-stabilized promoter driving phlF repressor; PPhlF DAPG-inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mr-GST full length

    Plasmid

    #112982

    Purpose

    For protein expression and purification of full-length Drosophila mr (APC2)

    Depositor

    Terry Orr-Weaver

    Insert

    mr (APC2) (mr Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-TRAM-CFP

    Plasmid

    #13027

    Depositor

    Doug Golenbock

    Insert

    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOpen-K12polLF (exo-)

    Plasmid

    #165522

    Purpose

    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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