We narrowed to 2,463 results for: T2A

1. eGFP Y93H AC editing reporter

   Plasmid

   #198883

   Purpose

   Express eGFP fluorescence after Y93H correction and mCherry as transfection control

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Y93H, Gly92(GGT-to-GGA)

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pREDIT_Cas9-MS2-BB_BbsI

   Plasmid

   #164802

   Purpose

   REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHLuc Control

   Plasmid

   #141091

   Purpose

   pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGLTR-X-PURO

   Plasmid

   #58246

   Purpose

   GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Insert

   Puro(R)
   

   Use

   Lentiviral and RNAi; Gateway destionation vector

   Tags

   TetR-NLS-T2A

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-eGFP-dSaCas9-blast

   Plasmid

   #191653

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   eGFP-T2A-dSaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. sgRNA3_GAL4UAS-Luciferase reporter

   Plasmid

   #64159

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQP-2473

   Plasmid

   #138976

   Purpose

   Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI
   

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST-HemmarI2

   Plasmid

   #112815

   Purpose

   gateway cloning destination vector for expressing enhancer driven CD4-IFP2.0-T2A-HO1

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDBEcR/Bla

   Plasmid

   #201556

   Purpose

   Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. eGFP Y93H GC editing reporter

    Plasmid

    #198884

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Y93H, Gly92(GGT-to-GGG)

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p-715MRPprom-MRPcucuc

    Plasmid

    #87199

    Purpose

    Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    RMRP (RMRP Human)
    

    Expression

    Mammalian

    Mutation

    GAGAG91-95 to CUCUC

    Promoter

    human RMRP

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sgRNA1_GAL4UAS-Luciferase reporter

    Plasmid

    #64157

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgRNA2_GAL4UAS-Luciferase reporter

    Plasmid

    #64158

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA2 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Antares-SEPLuc

    Plasmid

    #141086

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 66 to 91 on the Luciferas…

    Promoter

    CMV

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Bxb1-GA_AIO-TetOn_mScarlet_Convergent

    Plasmid

    #229790

    Purpose

    Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-MCS-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225704

    Purpose

    Empty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    T2A-mTurquoise

    Expression

    Mammalian

    Promoter

    pEF1A

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-MCS-3AID-HA-2A-mCherryBSD

    Plasmid

    #225700

    Purpose

    Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    T2A-mCherry

    Expression

    Mammalian

    Promoter

    pEF1A

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-rTetR(SE)-VP64-2A-mCherryBSD

    Plasmid

    #225695

    Purpose

    Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    rTetR-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mCherry

    Expression

    Mammalian

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psiT-GEM(0)

    Plasmid

    #220446

    Purpose

    Split intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4-CfaC
    

    Expression

    Insect

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psiT-GEM(2)

    Plasmid

    #220448

    Purpose

    Split intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4-CfaC
    

    Expression

    Insect

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge