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AdML-M3

Plasmid

#11244

Depositor

Robin Reed

Publication

Zhou et al Proc Natl Acad Sci U S A. 2002 Sep 17. 9

Insert

AdML-M3

Tags

3 MS2 hairpins

Expression

Bacterial

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
ABE8.8-m

Plasmid

#136294

Purpose

expresses ABE8.8-m in mammalian cells

Depositor

Nicole Gaudelli

Publication

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.8-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Nano-lantern(cAMP-1.6)/pcDNA3

Plasmid

#53594

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Publication

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Expression

Mammalian

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
p3662 HPV-16 E2

Plasmid

#22457

Depositor

Peter Howley

Publication

Sakai et al J Virol. 1996 Mar . 70(3):1602-11.

Insert

E2 WT

Expression

Mammalian

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-mGFP

Plasmid

#62629

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Publication

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSCC2

Plasmid

#39993

Depositor

Paul Modrich

Publication

Cheng et al J Biol Chem. 1984 Sep 25;259(18):11571-5

Insert

EcoRI endonuclease & EcoRI methylase

Tags

none

Expression

Bacterial

Mutation

none

Promoter

n/a

Available since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only

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hE-cadherin/Δp120

Plasmid

#45770

Depositor

Barry Gumbiner

Publication

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Δp120 (CDH1 Human)

Expression

Mammalian

Mutation

Amino acids 762-764 converted from EED to AAA to …

Promoter

CMV

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
HA-14-3-3 epsilon

Plasmid

#116886

Purpose

Expresses HA-tagged human 14-3-3 epsilon in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Publication

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 epsilon (YWHAE Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET28a-SpyTag002-MBP

Plasmid

#102831

Purpose

Bacterial expression of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Publication

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to the N-terminus of MBP via a …

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA3-V5-PTPN14-wild type

Plasmid

#61003

Purpose

Expresses wild type human PTPN14 in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Publication

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLentiPGK Hygro DEST H2B-mCerulean3

Plasmid

#90234

Purpose

Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Publication

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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p3993 pET ubcH7

Plasmid

#8654

Depositor

Peter Howley

Publication

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH7 (UBE2L3 Human)

Expression

Bacterial

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
UAS-mCherry

Plasmid

#135457

Purpose

Mammalian mCherry reporter to read out FLARE and SPARK activation

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry

Tags

mCherry

Expression

Mammalian

Promoter

UAS

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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p201H Cas9

Plasmid

#59176

Purpose

Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Publication

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
hph

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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PVS10

Plasmid

#104398

Purpose

polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Publication

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Str-KDEL_ManII-SBP-EGFP

Plasmid

#65252

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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AAV-hSyn-FlicR1

Plasmid

#74143

Purpose

Express FlicR1 preferentially in neurons

Depositor

Robert Campbell

Publication

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-8XPP7

Plasmid

#121940

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJ3 SHP1 C453S

Plasmid

#8573

Depositor

Ben Neel

Insert

SHP1 (PTPN6 Human)

Expression

Mammalian

Mutation

C453S (catalytically inactive)

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only