We narrowed to 2,983 results for: erf

Showing: 1821 - 1840 of 2983 results

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pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA

Plasmid

#108835

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Tags

cmyc tag

Expression

Yeast

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFDP1

Plasmid

#67084

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP1 (TFDP1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pGIPZ EF1a IRES miRFP720-P2A-Blast

Plasmid

#248029

Purpose

Backbone that constitutively expresses miRFP720 and a blasticidin resistance gene in human cells

Depositor

Joshua Leonard

Publication

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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TRF2-HA

Plasmid

#200209

Purpose

Expression of full length mTRF2

Depositor

Sheila Teves

Publication

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRF2-HA (Terf2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Publication

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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4xHRE_miniTK-sfGFP-CMV_dsRed Exp

Plasmid

#117404

Purpose

sfGFP expressed from hypoxia-inducible miniTK promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Publication

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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tdEos-VASP-5

Plasmid

#57685

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VASP

Tags

tdEos

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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PDZD3

Plasmid

#38880

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PDZD3 (PDZD3 Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_G12-E

Plasmid

#25761

Purpose

Entry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Gateway Cloning

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_TmiR_Gb2-K

Plasmid

#25764

Purpose

Entry vector with TRE promoter driving mouse G beta 2 miR30-based shRNA.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Gateway Cloning

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-TRIF(Nterm)

Plasmid

#221334

Purpose

Overexpression of TRIF Nterm domain in bacteria

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF Nterm domain (Ticam1 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-RFP(pLxIS-PLPLR)

Plasmid

#221284

Purpose

Expression of RFP (pLxIS-PLPLR) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

RFP (pLxIS-PLPLR) (Mkrn1 Synthetic)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt7

Plasmid

#213630

Purpose

Bacterial expression of SUMO-tagged mouse FL-syt7

Depositor

Edwin Chapman

Publication

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt7 (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pYZ694

Plasmid

#132956

Purpose

crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and nucleoplasmin NLS

Promoter

adh1

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pOmniBac zz TEV YBBR TRF1

Plasmid

#185445

Purpose

Expresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

Depositor

Ahmet Yildiz

Publication

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Insert

TRF1 (TERF1 Human)

Tags

YBBR and ZZ

Expression

Insect

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEN-R2-mCsfGFP-L3

Plasmid

#178602

Purpose

It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

Depositor

Frederica Theodoulou

Publication

Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

Insert

Tandem fluorescent protein timers (tFTs)

Use

Gateway Cloning

Tags

mCherry superfolder GFP

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-160

Plasmid

#74607

Purpose

Expresses Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Publication

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFDP3

Plasmid

#67083

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP3 (TFDP3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 GTF2H4

Plasmid

#67094

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H4 (GTF2H4 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only