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pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

Plasmid

#240229

Purpose

Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-spatzle-HA

Plasmid

#240235

Purpose

Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

spatzle (spz Fly)

Tags

HA-spGFP11

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_hhN-HA

Plasmid

#240225

Purpose

Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

hedgehog N-terminal fragment (hh Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Mutation

aa 1-257

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS140

Plasmid

#197141

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS191

Plasmid

#197140

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

EF1a

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pNM1325

Plasmid

#197144

Purpose

CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

SpCas9-PmCDA1-UGI

Use

CRISPR

Expression

Mammalian

Mutation

SpCas9(D10A)

Promoter

CAG

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-146

Plasmid

#197136

Purpose

CloneSelect Indel reporter encoding BC-del1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-196

Plasmid

#197138

Purpose

CloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-117

Plasmid

#197134

Purpose

CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSI-218

Plasmid

#197139

Purpose

All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

CRISPR

Tags

SpCas9-PmCDA1-UGI

Expression

Mammalian

Mutation

SpCas9(D10A)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-060

Plasmid

#197133

Purpose

CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

MCP(N55K)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-193

Plasmid

#197137

Purpose

CloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

mCherry(M1V/M9A)

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-119

Plasmid

#197135

Purpose

CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-RCaMP1h

Plasmid

#42874

Purpose

a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

Depositor

Loren Looger

Article

Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

Insert

Red Fluorescent Calcium binding Protein

Tags

EK (Enterokinase) Recognition Site, His Tag (6x),…

Expression

Bacterial

Promoter

T7

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#141210

Purpose

empty GFP vector for cloning sgRNA or pgRNAs

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP_AAVS1

Plasmid

#141211

Purpose

pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Insert

gRNA

Use

Lentiviral

Expression

Mammalian

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_AAVS1_1801

Plasmid

#159090

Purpose

targeting, non-essential locus control

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Insert

gRNA

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUAS-hhN-HA

Plasmid

#240236

Purpose

Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

hedgehog N-terminal fragment (hh Fly)

Tags

HA-spGFP11

Expression

Insect

Mutation

aa 1-257

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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240 pTol2005b

Plasmid

#73483

Purpose

Tol2 vector with multi cloning site

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Type

Empty backbone

Use

Tol2

Promoter

none

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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DBJS-p8.72

Plasmid

#246901

Purpose

Used for multi-site piggybac transposon insertion of MCP-tagged CAPRIN1

Depositor

David Bartel

Insert

MCP::eGFP::CAPRIN1 + Hygro resistance (CAPRIN1 Human)

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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