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We narrowed to 18,567 results for: puro

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Showing: 1821 - 1840 of 18567 results

  1. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid
    #217971
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.
    Depositor
    Robert Piper
    Publication
    Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.
    Insert
    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    Expression
    Mammalian
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    217971-plasmid-map-sequence-id-436106

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  2. pTS2354_Tier3(PB)-Ruby_PuroR

    Plasmid
    #169653
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mRuby2 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169653-plasmid-map-sequence-id-366195

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  3. ATM-Halo HRD

    Plasmid
    #207082
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207082-plasmid-map-sequence-id-430992

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  4. RNF168-Halo HRD

    Plasmid
    #207080
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Expression
    Mammalian
    Promoter
    None
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207080-plasmid-map-sequence-id-432323

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  6. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

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  7. pGMC00009 (aka pRC0494)

    Plasmid
    #195294
    Purpose
    Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195294-plasmid-map-sequence-id-385588

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  10. 3xMS-TR-100-PURO HRD

    Plasmid
    #207582
    Purpose
    homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus
    Depositor
    Jens Schmidt
    Publication
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Insert
    3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
    Tags
    3xMS2
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207582-plasmid-map-sequence-id-429467

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  11. pCDH-EF1S-Nluc

    Plasmid
    #73033
    Purpose
    Express Nluc
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    Truncated EF1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73033-plasmid-map-sequence-id-133974

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  12. FUGW-APOE-E4-T2A-Puro

    Plasmid
    #190810
    Purpose
    Express APOE E4 allele from Ubiquitin C promoter, co-expressing Puro resistance gene
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Mutation
    E4
    Promoter
    Ubi C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190810-plasmid-map-sequence-id-372856

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  13. pGMC00010 (aka pRC0495)

    Plasmid
    #195295
    Purpose
    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195295-plasmid-map-sequence-id-387224

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  14. pTet-O-APOE-E4-T2A-Puro

    Plasmid
    #190813
    Purpose
    Express APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance gene
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Mutation
    E4
    Promoter
    Tet-O
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190813-plasmid-map-sequence-id-372861

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  15. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid
    #196196
    Purpose
    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus
    Depositor
    Jürgen Knoblich
    Publication
    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75
    Insert
    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    Use
    Homology repair donor
    Tags
    V5-APEX2-mScarlet-I
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    196196-plasmid-map-sequence-id-385065

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  16. XLone-BSD NFIL3 P2A eGFP NLS

    Plasmid
    #140030
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 (NFIL3 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140030-plasmid-map-sequence-id-275866

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  17. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  18. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140032-plasmid-map-sequence-id-275878

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Showing: 1821 - 1840 of 18567 results