We narrowed to 6,452 results for: sas

1. pET32a-HD39Q

   Plasmid

   #11514

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 39 glutamines (HTT Human)
   

   Use

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 39 glutamines, vector m…

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pET32a-Spike-S-4P

   Plasmid

   #165094

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pSCT-GAL93-NONO

   Plasmid

   #46325

   Depositor

   Steven Brown

   Article

   Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

   Insert

   NONO (NONO Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pLJM1 FLCN (F118D) FLAG GFP

   Plasmid

   #164979

   Purpose

   Lentiviral vector for FLCN expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Use

   Lentiviral

   Tags

   1xFLAG-GFP

   Expression

   Mammalian

   Mutation

   F118D

   Promoter

   UBC

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

   Plasmid

   #98932

   Purpose

   Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FnoCas12a-2C-NLS in pCSDest

   Plasmid

   #126639

   Purpose

   Expresses FnoCas12a-2C-NLS in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnoCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94 promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LUC45 (L/Sec/mutP)

   Plasmid

   #112148

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U) and SE…

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJH3456

   Plasmid

   #179581

   Purpose

   Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   BFP

   Expression

   Worm

   Mutation

   Promoter

   Pnmr-1

   Available since

   March 2, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pLKO.1-puro U6 sgRNA Oct4A -158

   Plasmid

   #50922

   Purpose

   U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   Oct4A -158 sgRNA (POU5F1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET30a-SARS-CoV2-ORF3b (S23Q)

    Plasmid

    #165088

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Stop codon to glutamine at 23rd amino acid

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pLKO.1-puro U6 sgRNA Oct4A -12

    Plasmid

    #50921

    Purpose

    U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Oct4A -12 sgRNA (POU5F1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-NLC-DHFR-SpCas9

    Plasmid

    #124523

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NLC-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40

    Plasmid

    #98931

    Purpose

    Cre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    iGluSnFr
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

    Plasmid

    #156441

    Purpose

    For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA2 (Stag2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5 Flag Grb10 5A short

    Plasmid

    #37484

    Depositor

    David Sabatini

    Article

    Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

    Insert

    Grb10 5A (GRB10 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    isoform C (missing N-term 58aa), 536aa, S150A T15…

    Promoter

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-sACE2-WT(732)

    Plasmid

    #154098

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 (untagged)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCapV

    Plasmid

    #183028

    Purpose

    the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28

    Depositor

    Ute Romling

    Article

    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

    Insert

    patatin-like phospholipase CapV
    

    Use

    Tags

    No

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFRT-DHX36iso2E335A

    Plasmid

    #159588

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    E335A

    Promoter

    CMV

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDF1-ADAMTS18 Wt

    Plasmid

    #29552

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pET32a-Spike-403-505

    Plasmid

    #165095

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge