159,428 results

1. pEM-Cas9HF1-indRecA56

   Plasmid

   #102294

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE)

   Plasmid

   #214876

   Purpose

   Lentiviral vector encoding RfxCas13d nontargeting control guide

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crNT-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMycVec2

   Plasmid

   #32361

   Depositor

   Michael Niederweis

   Article

   Kaps et al Gene. 2001 Oct 31;278(1-2):115-24.

   Insert

   pJAZ38 ori
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-ERBB2

   Plasmid

   #116734

   Purpose

   Lentiviral expression of ERBB2

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304-VHL-IRES-GFP

   Plasmid

   #187641

   Purpose

   VHL Expression vector with GFP

   Depositor

   William Kaelin

   Article

   Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

   Insert

   VHL (VHL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pE-FLP

   Plasmid

   #45978

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Insert

   flp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   flp driven by the constitutive promoter pE from p…

   Promoter

   pE (from phage P2)

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-EFS-FLTID-GSQ-PMLivaWT-P2A-BLAST

   Plasmid

   #208040

   Purpose

   Enables constitutive expression of N-terminal TurboID-fused PML (isoform IVa; WT); selection with blasticidin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   PML (isoform IVa) (PML Human)
   

   Use

   Lentiviral

   Tags

   FLAG, TurboID

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 57F03_LexA-GAD

   Plasmid

   #83256

   Purpose

   Expresses LexA-GAD under the control of 57F03 enhancer

   Depositor

   Leslie Vosshall

   Article

   Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

   Insert

   57F03 enhancer
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC18-mini-Tn7T-Gm-LacZ10

   Plasmid

   #65026

   Purpose

   GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Type

   Empty backbone

   Use

   Tags

   lacZ

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

    Plasmid

    #216533

    Purpose

    An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

    Depositor

    Yuki Kambe

    Article

    Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

    Insert

    axon-jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    GAP43

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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