We narrowed to 34,820 results for: CaS;

1. pMA-SpCas9-g1

   Plasmid

   #80784

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPomyces-AsCas12j-2

   Plasmid

   #191655

   Purpose

   All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

   Depositor

   Fong Tian Wong

   Article

   Tan et al bioRxiv 2021.10.28.465406

   Insert

   AsCas12j-2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. His_CL7_2NLS_iCas12a-4NLS

   Plasmid

   #222971

   Purpose

   Bacterial expression of NLS-rich iCas12a construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

   Insert

   His_CL7_2NLS_iCas12a-4NLS
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SauKKHCas9 PE2*

   Plasmid

   #169852

   Purpose

   Mammalian Expression, sauCas9-KKH based prime editor

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SaCas9KKH-N580A-M-MLV
   

   Use

   Tags

   bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-dCas9-HA

   Plasmid

   #61355

   Purpose

   encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiGuide_CjCas9-Puro

   Plasmid

   #202564

   Purpose

   hU6-BsmBI-gRNA-Puro

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   CjCas9 sgRNA cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMA-SpCas9-g2

   Plasmid

   #80785

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEC1.2/EC1.1_Cas9i_TagRFP

   Plasmid

   #227249

   Purpose

   CRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP seed selection

   Depositor

   Christian Fankhauser

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pEC1.2/EC1.1

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH-nCas9-PPE

   Plasmid

   #140447

   Purpose

   For plant prime editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   nCas9(H840A)-M-MLV
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   H840A for Cas9; D200N, T306K, W313F, T330P and L…

   Promoter

   maize Ubiquitin-1, OsU3

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-T3-hCAS-pA

    Plasmid

    #48625

    Purpose

    Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Kunihiko Naito, Wataru Fujii

    Article

    Fujii et al Nucleic Acids Res. 2013 Aug 30.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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