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Showing: 2761 - 2790 of 20879 results

  1. pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

    Plasmid
    #126444
    Purpose
    Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated
    Depositor
    Jeremy Wilusz
    Article
    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:
    Insert
    Fndc3b (Fndc3b Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    circFndc3b exons are separated by a miniature int…
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126444-plasmid-map-sequence-id-245088

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  2. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118155
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    118155-plasmid-map-sequence-id-243704

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  3. TFORF1453

    Plasmid
    #143279
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DLX2 (DLX2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143279-plasmid-map-sequence-id-396135

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  5. 6-ISG-IFN

    Plasmid
    #231488
    Purpose
    Standard for type I interferon signature (6 genes)
    Depositor
    Stefano Volpi
    Article
    Tesser et al Clin Exp Immunol. 2025 Mar 24:uxaf018. d
    Inserts
    TBP (TBP Human)
    IFI27 (IFI27 Human)
    IFI44L (IFI44L Human)
    IFIT1 (IFIT1 Human)
    ISG15 (ISG15 Human)
    RSAD2 (RSAD2 Human)
    SIGLEC1 (SIGLEC1 Human)
    HPRT1 (HPRT1 Human)
    18s (RPS18 Human)
    G6PD (G6PD Human)
    Use
    Qpcr plasmid standard
    Tags
    Expression
    Mutation
    partial sequence
    Promoter
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231488-plasmid-map-sequence-id-469002

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  6. AiP12044 - pAAV-3xSP10ins-AiE0357h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2044)

    Plasmid
    #223973
    Purpose
    AiE0357h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical astrocytes (sparse)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    March 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    223973-plasmid-map-sequence-id-462737

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  7. pENTR4-SNAPf (w878-1)

    Plasmid
    #29652
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    SNAPf
    Expression
    Mutation
    Promoter
    Available since
    May 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    29652-plasmid-map-sequence-id-220455

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  8. pENTR4-CLIPf (w877-2)

    Plasmid
    #29650
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    CLIPf
    Expression
    Mutation
    Promoter
    Available since
    July 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    29650-plasmid-map-sequence-id-13765

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  10. pENTR4-HaloTag (w876-1)

    Plasmid
    #29644
    Depositor
    Eric Campeau
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    HaloTag
    Expression
    Mutation
    Promoter
    Available since
    May 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    29644-plasmid-map-sequence-id-220459

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  11. pAcGFP-IVT

    Plasmid
    #205145
    Purpose
    IVT template of AcGFP with MCS in frame and polyA
    Depositor
    Igor Ulitsky
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205145-plasmid-map-sequence-id-404881

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  12. pENTR4-SB1-LUC (w832-1)

    Plasmid
    #29647
    Depositor
    Eric Campeau
    Insert
    Firefly Luciferase
    Use
    Entry vector
    Tags
    SB1
    Expression
    Mutation
    Promoter
    Available since
    May 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    29647-plasmid-map-sequence-id-13762

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  13. pAcGFP-stop-IVT

    Plasmid
    #205146
    Purpose
    IVT template of AcGFP stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205146-plasmid-map-sequence-id-404879

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  14. pFFLuc-IVT

    Plasmid
    #205147
    Purpose
    IVT template of FireFly luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    FireFly luciferase
    Use
    Luciferase; In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205147-plasmid-map-sequence-id-404882

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  16. 3' EGFP pXOON

    Plasmid
    #48699
    Purpose
    Creates a 3' Fusion of EGFP to insert for expression in Xenopus oocytes or mammalian cells
    Depositor
    Dmitri Boudko
    Type
    Empty backbone
    Use
    Xenopus oocyte expression
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    48699-plasmid-map-sequence-id-70536

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  17. pRLuc-IVT

    Plasmid
    #205148
    Purpose
    IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    Renilla Luciferase
    Use
    Luciferase; In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205148-plasmid-map-sequence-id-404883

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  18. pCV019-catP

    Plasmid
    #232825
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - chloramphenicol
    Depositor
    Athanasios Typas
    Insert
    catP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232825-plasmid-map-sequence-id-462956

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  19. pCV020-tetQ

    Plasmid
    #232826
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - tetracycline
    Depositor
    Athanasios Typas
    Insert
    tetQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232826-plasmid-map-sequence-id-462958

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  20. pCV006-ermg

    Plasmid
    #232824
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - erythromycin
    Depositor
    Athanasios Typas
    Insert
    ermG
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232824-plasmid-map-sequence-id-462957

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  21. 371-A

    Plasmid
    #97026
    Purpose
    yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97026-plasmid-map-sequence-id-182505

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  22. 371-U

    Plasmid
    #97036
    Purpose
    MYFQSNA-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    MYFQSNA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97036-plasmid-map-sequence-id-182502

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  23. pOpen-THEAQpolA

    Plasmid
    #165512
    Purpose
    DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    Taq DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165512-plasmid-map-sequence-id-339400

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  24. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid
    #52421
    Purpose
    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry
    Depositor
    Bertrand Collet
    Insert
    Fusion NLS-EGFP-P2A-Cherry-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    52421-plasmid-map-sequence-id-81252

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  25. pOpen-T3gene1

    Plasmid
    #165576
    Purpose
    DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    T3 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165576-plasmid-map-sequence-id-340988

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  26. pKSB- 3xP3-DsRedSV40 GGGG AAGA

    Plasmid
    #174532
    Purpose
    3xP3-DsRed fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    3xP3-DsRed BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174532-plasmid-map-sequence-id-342920

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  27. pY126 (pcDNA3.1-huBoCpf1)

    Plasmid
    #92302
    Purpose
    Expression of humanized BoCpf1
    Depositor
    Feng Zhang
    Insert
    huBoCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92302-plasmid-map-sequence-id-181630

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  28. pUC-ODN2

    Plasmid
    #208643
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. Further remove BsaI and BsmBI site in pUC-ODN (Addgene #208642).
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208643-plasmid-map-sequence-id-418356

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  29. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Jen Sheen
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    58807-plasmid-map-sequence-id-91105

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  30. C35m

    Plasmid
    #120969
    Purpose
    MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).
    Depositor
    Richard Murray
    Insert
    RhlR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BbsI sites removed; amino acid sequence …
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120969-plasmid-map-sequence-id-238056

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Showing: 2761 - 2790 of 20879 results