We narrowed to 2,448 results for: ARA-2

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pAc5.1B-EGFP-DmGW182_635-1384_N

Plasmid

#147062

Purpose

Insect Expression of DmGW182_635-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_635-1384 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-41P-DmGW182_744-817_N

Plasmid

#147064

Purpose

Bacterial Expression of DmGW182_744-817

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_744-817 (gw Fly)

Expression

Bacterial

Mutation

one non silent mutations N799S and one silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_1-824_N

Plasmid

#147059

Purpose

Insect Expression of DmGW182_1-824

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_1-824 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA

Plasmid

#186407

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-pFOGc::RfA

Plasmid

#186397

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::RfA

Plasmid

#186396

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

Plasmid

#186413

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ E. coli)

Use

Gateway Cloning

Tags

Nuclear localization signal

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22gcfUbm5En25EE4PCh(#552)

Plasmid

#184092

Purpose

cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

5myc-chkEn2(5E)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Mutation

chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22Ubm4Eng2bKRPC(#493)

Plasmid

#184091

Purpose

cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

4xMyc

Mutation

ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pXalb_IF1_Cascade

Plasmid

#178768

Purpose

Expression of X. albilineansCascade type I-F1 Cascade genes

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

X. albilineansCascade type I-F1 Cascade genes

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pXalb_IF1_Cas2-3

Plasmid

#178769

Purpose

Expression of X. albilineans type I-F1 cas2-3

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

X. albilineans type I-F1 cas2-3

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJJF496_dpy-10_CDS_sg6

Plasmid

#164267

Purpose

sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the coding sequence of dpy-10

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF495_dpy-10_CDS_sg6

Plasmid

#164268

Purpose

sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the coding sequence of dpy-10

Use

CRISPR

Expression

Worm

Promoter

U6 promoter from W05B2.8

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF144_SECGFP_sg2

Plasmid

#163865

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF449_dpy-10_CDS_sg1

Plasmid

#163866

Purpose

sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the coding sequence of dpy-10

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF328_sqt-3_3′UTR_sg2

Plasmid

#163867

Purpose

sgRNA targeting sqt-3 (3′UTR) in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the 3'UTR of sqt-3

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF143_SECGFP_sg1

Plasmid

#163864

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4ΔCTD

Plasmid

#157800

Purpose

Expression of mEGFP fused to human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4ΔCTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+LANA-H1.4CTD

Plasmid

#157798

Purpose

Expression of LANA peptide-human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

LANA-H1.4CTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHis8-4b-McDAH

Plasmid

#140129

Purpose

Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Article

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase from Menispermum canadense

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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