We narrowed to 157,076 results for: Des

1. pdest METTL1-S27A Neo

   Plasmid

   #223059

   Purpose

   METTL1-S27A gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   METTL1-S27A (METTL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S27A

   Promoter

   CMV

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdest METTL1-S27D Neo

   Plasmid

   #223060

   Purpose

   METTL1-S27D gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   METTL1-S27D (METTL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S27D

   Promoter

   CMV

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdest myr-AKT2 Neo

   Plasmid

   #223063

   Purpose

   myr-AKT2 gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   myr-AKT2 (Akt2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-CMV-zdgat2FLAG-SV40

   Plasmid

   #232141

   Purpose

   Expresses zebrafish dgat2 with a C-terminal 3xFLAG tag under control of the CMV promoter

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat2 (dgat2 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   3xFLAG

   Promoter

   CMV

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest17_A1-LCD swap X10

   Plasmid

   #226552

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD X10

   Depositor

   Tanja Mittag

   Article

   Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

   Insert

   A1-LCD swap X10
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest-pol2-Smarcd1-Myc

   Plasmid

   #227716

   Purpose

   Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.

   Depositor

   Kent Hunter

   Article

   Ross et al bioRxiv 2024.01.24.577061

   Insert

   Smarcd1 (Smarcd1 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   pol2

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST17-ScChd1[R126A/R130A]

   Plasmid

   #221197

   Purpose

   ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   ScChd1 (aa118-1274)
   

   Tags

   6xHis-tag (Precission protease cleavable)

   Expression

   Bacterial

   Mutation

   R126A, R130A

   Promoter

   T7

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. N-SF-TAP-DEST-TLK2-D592V

   Plasmid

   #218835

   Purpose

   Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).

   Depositor

   Travis Stracker

   Insert

   TLK2 Q592V (kinase dead) (TLK2 Human)
   

   Tags

   FLAG-STREP

   Expression

   Mammalian

   Mutation

   Q592V

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDestTol2-QUAS:mApple-he1.1:CFP

   Plasmid

   #184812

   Purpose

   Used to generate the Tg(QUAS:mApple; he1.1:CFP)c788 transgenic line

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   QUAS:mApple-pA; he1.1:CFP
   

   Use

   Zebrafish tol2 transgenesis

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST17-His10-ccdB-SNAP

    Plasmid

    #166146

    Purpose

    Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Tags

    His10 and SNAP

    Expression

    Bacterial

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST17-His10-Smt3-ccdB

    Plasmid

    #166147

    Purpose

    Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Tags

    His10-Smt3

    Expression

    Bacterial

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST17-His10-DDX5(kf)-SNAP

    Plasmid

    #166152

    Purpose

    Expression of N. furzeri DDX5 in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5
    

    Tags

    His10 and SNAP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST14-Cys-SpyCatcher (Min)

    Plasmid

    #216713

    Purpose

    Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

    Depositor

    Alex Dunn

    Article

    Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

    Insert

    Cys-SpyCatcher (Min)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See Li...Howarth, J. Mol. Biol., 2014

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDESTpK7WG2-RPS5Ap-NLS-OleGFP

    Plasmid

    #193370

    Purpose

    nTALEN or nTALECD vector assembly, Destination vector for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

    Depositor

    Shin-ichi Arimura

    Article

    Hosoda et al bioRxiv 2023.02.27.530312

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDestTol2pA ins:Apollo-NADP+ mVenus

    Plasmid

    #210518

    Purpose

    Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

    Depositor

    Jonathan Rocheleau

    Article

    Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

    Insert

    Apollo-NADP+
    

    Use

    Zebrafish expression, tol2 kit gateway-compatible

    Tags

    mVenus

    Promoter

    Zebrafish insulin promoter

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTKEI-Dest-loxP-sfGFP

    Plasmid

    #202715

    Purpose

    Empty expression vector containing Cre recombinase reporter.

    Depositor

    Mary Dunlop

    Article

    Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

    Insert

    Pw7-loxP-term-loxP-sfGFP
    

    Use

    Cre/Lox

    Expression

    Bacterial

    Promoter

    constitutive

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMMACE DEST CMV PE2

    Plasmid

    #206276

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    PE2
    

    Use

    CRISPR; Recombinant baculovirus production, multi…

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-Blast-Dest-LifeAct-mRuby

    Plasmid

    #194907

    Purpose

    To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    LifeAct
    

    Use

    Mouse Targeting

    Tags

    mRuby

    Expression

    Mammalian

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-Myc-FBW7]K404R

    Plasmid

    #200715

    Purpose

    The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for overexpression and detection by western blotting.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 (FBXW7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FBW7 K404 is mutated to Alanine.

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-Myc-FBW7K404/412/R

    Plasmid

    #200716

    Purpose

    The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 & 412 to Arg mutation. Used for mammalian overexpression and detection by western blotting.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 (FBXW7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FBW7 K404 and K412 mutated to Alanines.

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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