We narrowed to 1,909 results for: TRIM

1. pCMV-N2-6MYC-hMID2

   Plasmid

   #51035

   Purpose

   mammalian expression of myc-tagged human MID2

   Depositor

   Timothy Cox

   Article

   Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

   Insert

   MID2 (MID2 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-myc-PDGFRalpha-Y206S

   Plasmid

   #136435

   Purpose

   Mammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant)

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   PDGFRalpha (PDGFRA Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Y206S (disrupts PDGF binding, but still binds HCM…

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry

   Plasmid

   #225584

   Purpose

   Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-F8.2 (S54L+T127A)-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T21R-F8.2 (S54L+T127A)

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p21-P369S-MEFV

   Plasmid

   #134712

   Purpose

   Lentiviral expression of human MEFV P369S after doxycycline administration

   Depositor

   Thomas Henry

   Article

   Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

   Insert

   MEFV (MEFV Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mutation

   p.P369S

   Promoter

   TRE2 (Dox-inducible)

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p21-M680I-MEFV

   Plasmid

   #134711

   Purpose

   Lentiviral expression of human MEFV M680I after doxycycline administration

   Depositor

   Thomas Henry

   Article

   Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

   Insert

   MEFV (MEFV Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mutation

   p.M680I

   Promoter

   TRE2 (Dox-inducible)

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p21-M694I-MEFV

   Plasmid

   #134710

   Purpose

   Lentiviral expression of human MEFV M694I after doxycycline administration

   Depositor

   Thomas Henry

   Article

   Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

   Insert

   MEFV (MEFV Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mutation

   p.M694I

   Promoter

   TRE2 (Dox-inducible)

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p21-S208C/M694V-MEFV

   Plasmid

   #134709

   Purpose

   Lentiviral expression of human MEFV S208C/M694V after doxycycline administration

   Depositor

   Thomas Henry

   Article

   Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

   Insert

   MEFV (MEFV Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mutation

   p.S208C/M694V

   Promoter

   TRE2 (Dox-inducible)

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p21-M694V-MEFV

   Plasmid

   #134703

   Purpose

   Lentiviral expression of human MEFV M694V after doxycycline administration

   Depositor

   Thomas Henry

   Article

   Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

   Insert

   MEFV (MEFV Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mutation

   p.M694V

   Promoter

   TRE2 (Dox-inducible)

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5/FRT/TO-GAlphai1-RLuc8

   Plasmid

   #140973

   Purpose

   Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphai1-RLuc8 (GNAI1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid

    #140982

    Purpose

    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaQ-RLuc8 (GNAQ Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX6P1‐hsRNASEH2BCA

    Plasmid

    #108692

    Purpose

    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

    Inserts

    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Shine Dalgarno sequence upstream of start codon a…

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-GGamma5-GFP2

    Plasmid

    #166777

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma5-GFP2 (GNG5 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-GGamma4-GFP2

    Plasmid

    #166776

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma4-GFP2 (GNG4 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Beta1

    Plasmid

    #140987

    Purpose

    Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta1 (GNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal human rhinovirus (HRV) 3C …

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

    Plasmid

    #208038

    Purpose

    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    PML (isoform IVa) (PML Human)
    

    Use

    Lentiviral

    Tags

    FLAG, N-terminal Split-TurboID

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid

    #140984

    Purpose

    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha15-RLuc8 (GNA15 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid

    #108693

    Purpose

    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

    Inserts

    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Shine Dalgarno sequence upstream of start codon, …

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

    Plasmid

    #140979

    Purpose

    Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaGustducin-RLuc8 (GNAT3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn T21R-F8.2 (S54L+T127A)-T2A-mCherry

    Plasmid

    #225590

    Purpose

    AAV transgene plasmid with hSyn promoter for expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-F8.2 (S54L+T127A)-T2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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