We narrowed to 5,293 results for: pcDNA3.1+

1. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

   Plasmid

   #114111

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

   Insert

   Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58

   Plasmid

   #171432

   Purpose

   Expression of hH3-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-hH3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/nV5-DEST-beta catenin

   Plasmid

   #20140

   Depositor

   Valeri Vasioukhin

   Article

   Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

   Insert

   pcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   T3I

   Promoter

   CMV

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/FGFR2-S252W(Apert) myc

   Plasmid

   #33249

   Depositor

   Alka Mansukhani, Claudio Basilico

   Article

   Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

   Insert

   FGFR2 (Fgfr2 Mouse)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Mutation S252W in FGFR2 found in Apert syndrome. …

   Promoter

   CMV

   Available since

   Dec. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1(+)-myc-OGA(GS-544)C181_NLS

   Plasmid

   #193997

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181_NLS

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181_NLS
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

   Plasmid

   #127314

   Purpose

   Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

   Plasmid

   #171427

   Purpose

   Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Pert Heavy chain-SpyTag003

   Plasmid

   #216309

   Purpose

   Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/CIP2A(1-905) L533E V5 His

   Plasmid

   #119288

   Purpose

   CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

   Depositor

   Jukka Westermarck

   Article

   Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

   Insert

   KIAA1524 (CIP2A Human)
   

   Use

   Tags

   V5 His

   Expression

   Mammalian

   Mutation

   L533E

   Promoter

   T7

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

    Plasmid

    #62975

    Purpose

    Expresses HA-tagged rat SOX9 residues 286-507

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA (YPYDVPDY)

    Expression

    Mammalian

    Mutation

    codes for residues 286-507

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

    Plasmid

    #163979

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 LIC 6A HIS-TEV-DPP9S

    Plasmid

    #166791

    Purpose

    Mammalian cell expression vector for His-tagged DPP9S

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1V5-His Snk/Plk2 T239D

    Plasmid

    #22220

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1 Flag-HIF1B [F446L] (#931)

    Plasmid

    #99917

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

    Insert

    HIF1B (ARNT Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F446L

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry

    Plasmid

    #181851

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    DnaJB1-PKAcat(K72H)-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lysine 128 in the DnaJB1-PKAcat fusion changed to…

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X

    Plasmid

    #147924

    Purpose

    Mammalian Expression of 6xbsMS2-Malat

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    6xbsMS2-Malat (Malat1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

    Plasmid

    #13005

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 delta CC (SULF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 87 to Alanine

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

    Plasmid

    #89636

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    S317A, AGC-->GCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 FLAG-SIV-RCM-vpx

    Plasmid

    #115844

    Purpose

    Encodes codon optimized FLAG tagged SIVRCM-Vpx

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVRCM-Vpx
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

    Plasmid

    #201217

    Purpose

    pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    Flag and SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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