165,688 results

1. pcDNA5FRT/Flag-WDR5

   Plasmid

   #29759

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   WDR5 (WDR5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 29, 2013

   Availability

   Academic Institutions and Nonprofits only

2. SPHK2 gRNA (BRDN0001145867)

   Plasmid

   #77094

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCh-Rab5

   Plasmid

   #49201

   Purpose

   expression of Rab5 fused to mCherry

   Depositor

   Gia Voeltz

   Article

   Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

   Insert

   Rab5 (RAB5B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-∆Hapln1

   Plasmid

   #200778

   Purpose

   Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
   

   Use

   AAV

   Tags

   cfGFP

   Mutation

   deleted lectican binding domain (aminoacids 40-15…

   Promoter

   Synapsin

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC-14-LEXA-QF

   Plasmid

   #46104

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   LexA-QF
   

   Expression

   Insect

   Available Since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. C1(1-29)-TurboID-V5_pLX304

   Plasmid

   #107175

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Lentiviral

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

   Plasmid

   #111903

   Purpose

   Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.

   Depositor

   Andrew Carter

   Inserts

   His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
   DYNC1I2 (DYNC1I2 Human)
   DYNC1LI2 (DYNC1LI2 Human)
   DYNLT1 (DYNLT1 Human)
   DYNLRB1 (DYNLRB1 Human)
   DYNLL1 (DYNLL1 Human)
   

   Tags

   8xHis, SNAPf-tag, TEV site, and ZZ-tag

   Expression

   Insect

   Mutation

   Optimised for expression in Sf9 cells.

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PL452-hGH-polyA-PGK-neoR

   Plasmid

   #191681

   Purpose

   Donor plasmid backbone PGK-neoR

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Type

   Empty backbone

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-Merlin S518A

   Plasmid

   #84294

   Purpose

   Expression of Merlin with an alanine mutation at the Pak phosphorylation site

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Merlin (NF2) (NF2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S518A

   Promoter

   CMV

   Available Since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-T2A-moxMaple3

    Plasmid

    #120875

    Purpose

    Mammalian expression of mCherry and moxMaple3

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

    Inserts

    mCherry
    moxMaple3
    

    Expression

    Mammalian

    Mutation

    from mMaple3, moxMaple3 was engineered by replaci…

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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