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pRK5-myc-RhoA-wt

Plasmid

#12962

Depositor

Gary Bokoch

Insert

RhoA (RHOA Human)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pSMT3_SRSF1

Plasmid

#201056

Purpose

Express sumo-tagged human SRSF1

Depositor

Jun Zhang

Article

Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

Insert

SRSF1 (SRSF1 Human)

Tags

sumo

Expression

Bacterial

Mutation

none

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC

Plasmid

#62806

Purpose

To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

Depositor

Jae Lee, Pantelis Tsoulfas

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Type

Empty backbone

Use

AAV; Eucaryotic

Expression

Mammalian

Promoter

hUbC

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2) cloning backbone

Plasmid

#61424

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-Cry2olig

Plasmid

#208780

Purpose

Inducibly expresses light activatable Cry2 domain

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cry2 mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S CK2M EN2L

Plasmid

#20924

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLA1

Plasmid

#160807

Purpose

Express POLA1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLA1 (POLA1 Human)

Use

Retroviral

Mutation

Codon Optimized

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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mCD8-GFP in pUAST

Plasmid

#17746

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

mouse CD8 (Cd8a Mouse)

Tags

GFP

Expression

Insect

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST47-SAR1-GFP

Plasmid

#67409

Purpose

Mammalian expression of hSar1 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
Lenti GFP-AMOT p80

Plasmid

#32830

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p80 (AMOT Human)

Use

Lentiviral

Tags

GFP-AMOT

Expression

Mammalian

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only