We narrowed to 19,480 results for: Tec

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pLEX307-Bat-CoV-HKU9-3CL

Plasmid

#160284

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMDC43-FIT2

Plasmid

#96993

Purpose

Express GFP-tagged mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Tags

GFP

Expression

Plant

Mutation

E2D (please see depositor comments below)

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

Plasmid

#123330

Purpose

Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-AKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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STK11 gRNA (BRDN0001147319)

Plasmid

#75914

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK11

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK11 (STK11 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

Plasmid

#160624

Purpose

TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:MS2:VPR:Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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TBK1 gRNA (BRDN0001148392)

Plasmid

#76362

Purpose

3rd generation lentiviral gRNA plasmid targeting human TBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TBK1 (TBK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pINTO-NFH::mEzh2

Plasmid

#65925

Purpose

Expression of mouse Ezh2 fused to Flag-HA tag (N-terminus)

Depositor

Roberto Bonasio

Article

Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

Insert

Enhancer of zeste homolog 2 (Ezh2 Mouse)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV/TO (T-REx) and CMV/TetO2 (T-REx)

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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CLXSN 5myc-CDC6-mut

Plasmid

#109333

Purpose

express tagged Cdc6 mutant

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

CDC6 (CDC6 Human)

Use

Retroviral

Tags

5x Myc

Expression

Mammalian

Mutation

R56A, L59A, K81A, E82A, N83A

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-HLA-c-myc-optiT1R3 ECD

Plasmid

#113960

Purpose

mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 21-563

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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TBK1 gRNA (BRDN0001145663)

Plasmid

#76361

Purpose

3rd generation lentiviral gRNA plasmid targeting human TBK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TBK1 (TBK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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