We narrowed to 34,812 results for: CaS;

1. pdCas12a(RuvC)

   Plasmid

   #186447

   Purpose

   Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   dCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D917A, E1006A

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SadCas9 KRAB

   Plasmid

   #188504

   Purpose

   Expresses FLAG tagged SadCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p276 eSpCas9_2gRNAs_hH11

   Plasmid

   #164850

   Purpose

   gRNA vector for targeting human H11 locus

   Depositor

   Philip Jordan

   Article

   Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

   Insert

   gRNAs for targeting human H11 locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNOC_dfnCas12a-KRAB_crRNA NC

   Plasmid

   #176262

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   Kruppel associated box domain and luciferase

   Expression

   Mutation

   D917A and E1006A mutations to inactivate the endo…

   Promoter

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiRCas9-CUG

   Plasmid

   #104183

   Purpose

   Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

   Depositor

   Eugene Yeo

   Article

   Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

   Insert

   U6-CUGsgRNA, EFS-PIN-dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. act5c-LbCas12a

   Plasmid

   #140620

   Purpose

   Ubiquitous expression of LbCas12a in Drosophila

   Depositor

   Michael Boutros

   Article

   Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-CBE6a

   Plasmid

   #215819

   Purpose

   Express CBE6a (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a-SpCas9-2xUGI (cas9 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-NRTH

   Plasmid

   #198541

   Purpose

   Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRTH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET_StrepII_TEV_LIC_TniQ(PmcCAST)

   Plasmid

   #224927

   Purpose

   TniQ expression plasmid for the co-purification of TnsC-TniQ complex

   Depositor

   Leifu Chang

   Article

   Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

   Insert

   TniQ
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. p426_Cas9_gRNA-YPRCd15c

    Plasmid

    #87404

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YPRCd15c sequence AATCCGAACAACAGAGCATA in yeast chromosome 14.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting YPRCd15c
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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