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Plasmid#89480PurposeEncodes for a fusion protein between a mitochondrial anchor and the CIBN-GFP protein.DepositorInsertmito-CIBN-GFP (CIB1 Mustard Weed, Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CRY2-mCh-superPLDx10
Plasmid#188986PurposeA lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)DepositorInsertPLD
UseTagsCRY2 and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP-SspBx6
Plasmid#103778Purposeexpression of iPOLYMER component in mammalian cellsDepositorInsertYFP-SspBx6
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD-CRY2PHR
Plasmid#28244PurposeExpresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activationDepositorInsertcry2 (CRY2 Mustard Weed)
UseTagsGal4BDExpressionYeastMutationPromoterAvailable sinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD-CRY2
Plasmid#28243PurposeExpresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activationDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGal4BDExpressionYeastMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CRY2-mCh-superPLDx30
Plasmid#188988PurposeA lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)DepositorInsertPLD
UseTagsCRY2 and mCherryExpressionMammalianMutationK109R, P245A, G328S, G381V, G429DPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-tTA
Plasmid#175280PurposeAAV vector mediating neuron-specific expression of tTADepositorInserttTA advanced (improved tetracycline-controlled transactivator)
UseAAVTagsExpressionMutationPromoterSynapsinAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry::LANS4
Plasmid#60785PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear TranslocationDepositorInsertLANS4
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIB1(deltaNLS)-pmGFP
Plasmid#28240PurposeExpresses CIB1 fused to plasma membrane-localized EGFPDepositorInsertCIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
UseTagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-CaM-NanoLuc-15aa-uTEVp
Plasmid#163034PurposeFLiCRE protease with luciferin-gating for neuronal expressionDepositorInsertGFP-CaM-NanoLuc-15aa-uTEVp
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Micro
Plasmid#60410PurposeE. coli expression of SspB MicroDepositorInsertSspB micro
UseTagsHis-MBP-TEVExpressionBacterialMutationR73Q, Y11K and A15EPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP
Plasmid#32481PurposeCre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertPSAML141F,Y115F-GlyR
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_FRT_TO_TurboID-mCherry-Cry2
Plasmid#166504PurposeExpression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.DepositorInsertNone
UseTagsTurboID and mCherry-CRY2ExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-OptoPB
Plasmid#113935PurposeExpress a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactionsDepositorInsertOptoPB
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDBTrp-LexABD-CRY2FL
Plasmid#78210PurposeUse with Addgene 40615 for blue light transcription control in yeastDepositorInsertLexABD-CRY2 (CRY2 Mustard Weed)
UseTagslexA binding domainExpressionYeastMutationcontains endogenous CRY2 NLSPromoterADH1Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA
Plasmid#74513PurposeAAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.DepositorInsertEGFP-p2A-EGFP-f
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2-IRES-dOrai
Plasmid#67627PurposeMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOraiDepositorInsertdmBACCS2-IRES-dOrai
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-synp-F-H2B-jRGECO1a
Plasmid#102995PurposeExpresses histone fused jRGECO1a under the synapsin promoterDepositorInsertH2B-jRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L iLID (C530M)
Plasmid#60408PurposeE. coli expression of iLID (C530M)DepositorInsertiLID C530M
UseTagsHisExpressionBacterialMutationC530MPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only