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Plasmid#183088Purpose2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assemblyDepositorInsertE2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEDJ506
Plasmid#177274PurposeADH1t:HIS3_23∆29-XII-5 :<-PGK1pDepositorInsertADH1t:HIS3_23∆29-XII-5 :<-PGK1p
UseTagsExpressionBacterial and YeastMutationPromoterPGK1Available sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-PGAL7-A_GFP_TCYC1
Plasmid#177162PurposeExpresses yeGFP under the control of a synthetic promoter (PGAL7-A)DepositorInsertyeGFP
UseTagsExpressionYeastMutationPromoterSynthetic promoter (PGAL7-A)Available sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-PGAL7-B_GFP_TCYC1
Plasmid#177163PurposeExpresses yeGFP under the control of a synthetic promoter (PGAL7-B)DepositorInsertyeGFP
UseTagsExpressionYeastMutationPromoterSynthetic promoter (PGAL7-B)Available sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-PGAL7-C_RFP_TCYC1
Plasmid#177164PurposeExpresses yeRFP under the control of a synthetic promoter (PGAL7-C)DepositorInsertyeCherry
UseTagsExpressionYeastMutationPromoterSynthetic promoter (PGAL7-C)Available sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-PGAL7-D_RFP_TCYC1
Plasmid#177165PurposeExpresses yeRFP under the control of a synthetic promoter (PGAL7-D)DepositorInsertyeCherry
UseTagsExpressionYeastMutationPromoterSynthetic promoter (PGAL7-D)Available sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEDJ508
Plasmid#177275PurposeADH1t:T7p:cgRNA_HIS3_stop:tZDepositorInsertADH1t:T7p:cgRNA_HIS3_stop:tZ
UseTagsExpressionBacterial and YeastMutationPromoterN/AAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyterm-mTq2 pDRF1-GW
Plasmid#168078PurposeExpresses Cyterm-mTq2 to check for monomericity.DepositorInsertcyterm-mTq2
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyterm-ymVenus pDRF1-GW
Plasmid#168079PurposeExpresses Cyterm-mVenus to check for monomericity.DepositorInsertcyterm-mVenus
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyterm-yeVenus pDRF1-GW
Plasmid#168080PurposeExpresses Cyterm-yeVenus to check for monomericity.DepositorInsertcyterm-yeVenus
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyterm-tagRFPT pDRF1-GW
Plasmid#168081PurposeExpresses Cyterm-tagRFPT to check for monomericity.DepositorInsertcyterm-tagRFPT
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIR3DH8
Plasmid#165068PurposeKnocking out GAL80 using KlURA3 markerDepositorInsertgal80Arm1-P-AgTEF1-KlURA3-T-AgTEF1-gal80Arm2
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR-wt
Plasmid#153339PurposeWild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoterDepositorInsertVanR
UseTagsExpressionYeastMutationPromoterTEF1Available sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-ECM33_intron
Plasmid#127598Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3DepositorInsertECM33 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-no_intron
Plasmid#127597Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoterDepositorInsert1xFLAG-MCP expression cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV23
Plasmid#128887PurposePosition 4 module- STOP (stop codon)DepositorInsertSTOP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV20
Plasmid#128879PurposePosition 2 module- Cyc1 promoterDepositorInsertCyc1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV19
Plasmid#128878PurposePosition 2 module- Adh1 promoterDepositorInsertAdh1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV17
Plasmid#128877PurposePosition 2 module- Act1 promoterDepositorInsertAct1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV2
Plasmid#128876PurposePosition 2 module- Met25 promoterDepositorInsertMet25 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV14
Plasmid#128861PurposePosition 1 module -2 micronsDepositorInsert2 microns
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV45
Plasmid#128885PurposePosition 3 module- 3SV40NLS (3x NLS from SV40)DepositorInsertSV40 NLS
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV48
Plasmid#128881PurposePosition 2 module- Tdh3 promoterDepositorInsertTdh3 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV1
Plasmid#128867PurposePosition 2 module- Gal1 promoterDepositorInsertGal1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMV6
Plasmid#128890PurposePosition 5 module- Adh1 terminatorDepositorInsertADH1 terminator
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV5
Plasmid#128886PurposePosition 4 module- CLN2-PEST (Degron)DepositorInsertCLN2-PEST
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV21
Plasmid#128880PurposePosition 2 module- Tef1 promoterDepositorInsertTef1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW YPET-T2A-mTurquoise2
Plasmid#118450PurposeProduces equimolar expression of YPET and mTq2DepositorInsertYPET-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yeVenus-T2A-mTurquoise2
Plasmid#118440PurposeProduces equimolar expression of yeVenus and mTq2DepositorInsertyeVenus-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only