We narrowed to 12,311 results for: BASE
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pHelper-CRISPRi-sgRNA
Plasmid#221135PurposeHelper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.DepositorInsertsgRNA-scaffold with dCas9 handle
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119(SpeI)Available sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-KO-PGK-Blast
Plasmid#227265PurposeEmpty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG66
Plasmid#226751PurposeExpresses exogenous GFPDepositorInsertGFP
UseLentiviralTagssfGFPExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)
Plasmid#225443PurposeGeminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.DepositorInsertBeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-ATP
Plasmid#225247PurposeBacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imagingDepositorInsertqmTQ2-ATP
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-glucose
Plasmid#225252PurposeBacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucoseDepositorInsertqmTQ2-glucose
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-cAMP
Plasmid#225249PurposeBacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP4
Plasmid#209959PurposeEntry vector to store Level 0* partsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP5
Plasmid#209960PurposeEntry vector to store Level 0* resistance cassette partsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP6
Plasmid#209961PurposeEntry vector to store Level 0* replication origin partsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(1-157)
Plasmid#220743PurposeGAL4-sfGFP(1-157) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(1-157)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(158-238)
Plasmid#220744PurposeGAL4-sfGFP(158-238) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(158-238)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-NIR-FbLAG30
Plasmid#220746PurposeGAL4-NIR-FbLAG30 construct expression in mammlian cells.DepositorInsertGAL4-NIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG16
Plasmid#220739PurposeNIR-FbLAG16 expression in mammalian cells.DepositorInsertNIR-FbLAG16
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG30
Plasmid#220740PurposeNIR-FbLAG30 expression in mammalian cells.DepositorInsertNIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb(718)
Plasmid#220742PurposeNIR-Fb(718) expression in mammalian cells.DepositorInsertNIR-Fb(718)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM042
Plasmid#216812PurposeAspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmScarlet3
UseTagsVinnyLinkerExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM043
Plasmid#216813PurposeAspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.DepositorInsertmScarlet3
UseTagsVinnyLinkerExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM052
Plasmid#216814PurposeAspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmNeonGreen
UseTagsVinnyLinkerExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM053
Plasmid#216815PurposeAspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.DepositorInsertmNeonGreen
UseTagsRIPGLINExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM070
Plasmid#216816PurposeAspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmCardinal
UseTagsVinnyLinkerExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM071
Plasmid#216817PurposeAspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.DepositorInsertmCardinal
UseTagsRIPGLINExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
2COX8-BmTyr
Plasmid#222943Purposemitochondrion-localized BmTyrDepositorInsert2COX8-BmTyr-Flag
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
IgK-BmTyr-KDEL
Plasmid#222942PurposeER-localized BmTyrDepositorInsertIgK-BmTyr-Flag-KDEL
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BmTyr-H2B
Plasmid#222941PurposeH2B-BmTyr fusionDepositorInsertBmTyr-H2B
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSRK51
Plasmid#223722PurposeExpresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
UseTagsNT* (self cleaving)ExpressionBacterialMutationL56V/S135G/S219VPromoterT7Available sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK469 (mAID-dTAG BSD)
Plasmid#214395PurposemAID-dTAG for C-terminal taggingDepositorInsertmAID-dTAG-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK484 (2mAID-Hygro)
Plasmid#214396Purpose2mAID for N-terminal taggingDepositorInsert2mAID-Hygro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK487 (Hygro-P2A-3mAID)
Plasmid#214399Purpose3mAID for C-terminal taggingDepositorInsertHygro-P2A-3mAID
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK450 (BromoTag-mCherry-BSD)
Plasmid#214380PurposeBromoTag-mCherry for N-terminal taggingDepositorInsertBromoTag-mCherry-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK454 (dTAG-BSD)
Plasmid#214384PurposedTAG for C-terminal taggingDepositorInsertdTAG-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR91
Plasmid#203915Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locusDepositorInsertCRF1 plus flanking (CRF1 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR90
Plasmid#203914Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locusDepositorInsertGSC2 plus flanking (GSC2 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR89
Plasmid#203423Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locusDepositorInsertEGC3 plus flanking (EGC3 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL6000
Plasmid#216797PurposePlatform for DiCre inducible expression of a gene of interest in Leishmania major - requires Leishmania strain expressing DiCre recombinaseDepositorTypeEmpty backboneUseCre/Lox; Leishmania dicre inducible expressionTags6xHAExpressionMutationPromoterrRNA promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p500-Erg6-V5-APEX2-URA
Plasmid#207070PurposeExpressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.DepositorInsertErg6
UseTagsV5-APEX2ExpressionYeastMutationPromoterpERG6Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-GFP-Pex3-4V5-APEX2-URA
Plasmid#207064PurposeExpressing GFP-Pex3-4V5-APEX2 under ADH3 promoter. Insufficient signal for EM.DepositorInsertPex3
UseTags4xV5-APEX2 and GFPExpressionYeastMutationPromoterpADH3Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-OM14-V5-APEX2-Ura
Plasmid#207073PurposeExpressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.DepositorInsertOM14
UseTagsV5-APEX2ExpressionYeastMutationPromoterpOM14Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL661-WT(TTTT)
Plasmid#215864PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL661 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only
