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Plasmid#225443PurposeGeminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.DepositorInsertBeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPU-HSA-miR30-L1221
Plasmid#174221PurposeAll-in-one control rescue-control shRNA knockdown vectorDepositorInsertHSA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK- TurboID- NCAN IL-HA
Plasmid#219808PurposeSecreted HA-tagged TurboID-NCAN-IL domain for extracellular proximity labelingDepositorInsertIgK (Igk Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL475
Plasmid#219503PurposeExpress Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in transDepositorInsertAde2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-eGFP-SAPAP3
Plasmid#176853PurposeExpresses SAPAP3 protein fused with eGFP at the N-terminus in neuronsDepositorInserteGFP-SAPAP3
UseAAVTagsGFPExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLRX-roGFP2 in pT3TS-Dest
Plasmid#194297PurposeIn vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRKDepositorInsertGLRX-roGFP2
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.407
Plasmid#187204PurposeExpresses retron Eco1 ncRNA v32, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v32
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.408
Plasmid#187205PurposeExpresses retron Eco1 ncRNA v35, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KG#920
Plasmid#110937PurposeExpresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animalDepositorInsertsmig-13 promoter
INS-22-Emerald
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM0649
Plasmid#129010PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM0655
Plasmid#129016PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporterDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0650
Plasmid#129011PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0648
Plasmid#129009PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-sod2 guide
Plasmid#107604PurposeAn entry vector with U6a and U6c promoter driving sod2 guide RNAs expressionDepositorInsertsod2 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-sod1 guide
Plasmid#107603PurposeAn entry vector with U6a and U6c promoter driving sod1 guide RNAs expressionDepositorInsertsod1 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-uqcrc1 guide
Plasmid#107602PurposeAn entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expressionDepositorInsertuqcrc1 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mus-Bnip3_deltaExon3
Plasmid#100793PurposeMammalian expression of Bnip3_deltaExon3 splice variantDepositorInsertmouse-Bnip3_deltaExon3 (Bnip3 Mouse)
UseTagsHAExpressionMammalianMutationExon3 deletionPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-3xFLAG-OB D166R
Plasmid#79061PurposeRetroviral expression of 3xFLAG tagged, nuclear localized TPP1-OB-RR folding domainDepositorInsertTPP1 (TPP1 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationAspartic acid 166 changed to ArgininePromoterCMVAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-mTmod3-150-352_domain
Plasmid#70755Purposemouse Tropomodulin3, 150-352 domain for bacterial expressionDepositorInsertTropomodulin3 150-352 AA fragment (Tmod3 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R3 #BGV148
Plasmid#48942PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR3 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only