We narrowed to 29,405 results for: des.2

1. pKLV2-U6gRNA5(ETV4_5-2)-PGKpuroBFP-W

   Plasmid

   #211961

   Purpose

   Express gRNA against ETV4 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting ETV4 (ETV4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169162

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
   

   Tags

   6His-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

   Plasmid

   #169163

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
   

   Tags

   3xFlag-6His

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pαH-S-GSAS-OMICRON.BA.2-RBD

   Plasmid

   #184830

   Purpose

   Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

   Depositor

   Priyamvada Acharya

   Article

   Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

   Insert

   S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6X His tag

   Expression

   Mammalian

   Mutation

   SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TMEM230-M4/X184PG-IRES(isoform 2)

   Plasmid

   #99568

   Purpose

   mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Expression

   Mammalian

   Mutation

   M4/X184PG

   Promoter

   CMV

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TMEM230-M3/Y92C-IRES(isoform 2)

   Plasmid

   #99567

   Purpose

   mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Expression

   Mammalian

   Mutation

   M3/Y92C

   Promoter

   CMV

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TMEM230-M2/X184W-IRES(isoform 2)

   Plasmid

   #99566

   Purpose

   mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Expression

   Mammalian

   Mutation

   M2/X184W

   Promoter

   CMV

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TMEM230-M1/R141L-IRES(isoform 2)

   Plasmid

   #99565

   Purpose

   mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Expression

   Mammalian

   Mutation

   M1/R141L

   Promoter

   CMV

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX_307-SARS-CoV-2-NSP3-V5

   Plasmid

   #214429

   Purpose

   Lentiviral-mediated stable expression of SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Mohan Babu

   Article

   Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

   Insert

   SARS-CoV-2 NSP3 (part of SARS-CoV-2 ORF1ab) (ORF1ab )
   

   Use

   Lentiviral

   Tags

   V5 tag

   Expression

   Mammalian

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

    Plasmid

    #159814

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

    Plasmid

    #159815

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTYB3-human PUM1-HD MUT6-2 7-2 (N1043S-Q1047E-S1079N-E1083Q)-intein-CBD

    Plasmid

    #73300

    Purpose

    Expresses mutant human PUM1-HD

    Depositor

    Traci Hall

    Article

    Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    human PUM1 Pumilio homology domain (PUM1 Human)
    

    Tags

    intein

    Expression

    Bacterial

    Mutation

    N1043S-Q1047E-S1079N-E1083Q

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159861

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159848

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159852

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

    Plasmid

    #197454

    Purpose

    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 Delta-WD40 propeller (FBXW7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FBW& dimerization domain deleted.

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS SARS-CoV-2 BQ.1.1 Spike

    Plasmid

    #193710

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BQ.1.1 Spike (S )
    

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19I, LPPA24S, …

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    Nov. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCAGGS SARS-CoV-2 BA.2.75 Spike

    Plasmid

    #190012

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BA.2.75 Spike (S )
    

    Expression

    Mammalian

    Mutation

    Contains the BA.2 mutations (T19I, LPPA24S, G142D…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    Sept. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCAGGS SARS-CoV-2 BA.4.6 Spike

    Plasmid

    #190013

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.4.6 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BA.4.6 Spike (S )
    

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19I, LPPA24S, …

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    Sept. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCAGGS SARS-CoV-2 BA.1 Spike

    Plasmid

    #185452

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike

    Depositor

    Marceline Côté

    Article

    Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:

    Insert

    SARS-CoV-2 Spike BA.1 (S )
    

    Expression

    Mammalian

    Mutation

    Contains the following mutations: A67V, Δ69-70, T…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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