We narrowed to 5,380 results for: kras

1. pAT9650-BEAR-GFP-preedited

   Plasmid

   #162992

   Purpose

   BEAR control plasmid with split EGFP and intact 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMV_AA088

   Plasmid

   #216104

   Purpose

   Knockout, EF1a-driven Cas9 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-GBP1-10gly-Gal4DBD

   Plasmid

   #49438

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP1-Gal4 DNA binding domain fusion protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C98S (to increase protein solubility), Q3D

   Promoter

   CAG

   Available since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR221-NFAT5

   Plasmid

   #79506

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   NFAT5 (NFAT5 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

   Plasmid

   #124861

   Purpose

   Mutagenesis of Slc17a8

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a8 (Slc17a8 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDP085

   Plasmid

   #198425

   Purpose

   pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120

   Depositor

   Markus Schmid

   Article

   You et al Nat Commun. 2017 May 17;8:15120. doi: 10

   Insert

   E. coli BirA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.0_LynLAMA-G97 IRES EGFP

   Plasmid

   #130711

   Purpose

   Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP
   

   Use

   Tags

   Lyn11 and SNAP-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-mCherry-C1-PsACR1

   Plasmid

   #85465

   Purpose

   Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)

   Depositor

   Peter Hegemann

   Article

   Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

   Insert

   Synthetic construct PsChR1 gene
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+enhancer)

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) R-Ras1(WT)

   Plasmid

   #224285

   Purpose

   Cell transfection and expression of R-Ras1

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   R-Ras1 (RRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2T-CAG-MCS-P2A-GFP-PuroR

    Plasmid

    #107186

    Purpose

    Base plasmid for cloning gene duplication alleles with eGFP reporter.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMV_AA090

    Plasmid

    #216099

    Purpose

    Knockout, EF1a-driven Cas12a (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas12a [EnAs]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3.0_LynLAMA-F98 IRES EGFP

    Plasmid

    #130710

    Purpose

    Plasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    Lyn11-SNAP-Tag-LAMA-F98 IRES EGFP
    

    Use

    Tags

    Lyn11 and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PXPR007 sgGFP-1

    Plasmid

    #74960

    Purpose

    Cas9 + sgGFP-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AA009

    Plasmid

    #216037

    Purpose

    Fragmid fragment: (C' terminus) Nucleoplasmin NLS

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    NpNLS_v1.1; 3xHA_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AA345

    Plasmid

    #216031

    Purpose

    Fragmid fragment: (Cas protein) Cas13d

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas13d_v1.1 [Dj]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_834

    Plasmid

    #216087

    Purpose

    Cas9 [Sp] knockout targeting CD59, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD59 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pG4-CMV-luc

    Plasmid

    #46322

    Depositor

    Steven Brown

    Article

    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

    Insert

    5xGal4
    

    Use

    Luciferase

    Tags

    CMV promoter and luciferase

    Expression

    Mammalian

    Mutation

    five copies of a multimerized GAL4 (5×GAL4) site …

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

18. eNme2-C TadCBEa

    Plasmid

    #193840

    Purpose

    Express TadCBEa (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadCBEa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA_Axin1 in pRK5

    Plasmid

    #154219

    Purpose

    N-terminal HA-tagged AXIN1 cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    AXIN1 (AXIN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGTag-P2A-QFGal4-SV40pA

    Plasmid

    #155127

    Purpose

    Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    P2A-QFGal4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge