We narrowed to 4,337 results for: Pet2

1. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

   Plasmid

   #167849

   Purpose

   Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp10, nsp16
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 22, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET28b – E. coli RecE residues 564-868

   Plasmid

   #104534

   Purpose

   Expresses E. coli RecE residues 564-868

   Depositor

   Charles Bell

   Article

   Zhang et al Structure. 2009 May 13;17(5):690-702. do

   Insert

   RecE residues 564-866 (C-terminal nuclease domain)
   

   Tags

   6X-His tag followed by thrombin site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21 Catalytic Domain of MALT1 Catalytic Mutant

   Plasmid

   #48971

   Purpose

   Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 (MALT1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains AA 329-566; Catalytic Mutant C464A

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

   Plasmid

   #175784

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Tags

   AviTag, Hexahistidine tag, and eGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

   Plasmid

   #153446

   Purpose

   E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.CohE
   

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-His6-OAZ-GSY-SPM-Ctag

   Plasmid

   #163613

   Purpose

   Expresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.

   Depositor

   Mark Howarth

   Article

   Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

   Insert

   OAZ-GSY-SPM
   

   Tags

   C-tag and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Kv7.4 AB (325-557, Δ 368-523)/pET28HMT

   Plasmid

   #111052

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Tags

   His Tag and MBP tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AB (325-557, Δ 368-523)

   Promoter

   T7

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-10XHis-GST-HRV-Her1-dL5

   Plasmid

   #73216

   Purpose

   Bacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

   Insert

   10XHis-GST-HRV-Her1-dL5 (EGFR Human, Synthetic, Schistosoma japonicum)
   

   Tags

   10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …

   Expression

   Bacterial

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   T7

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-10XHis-GST-HRV-dL5-Her1

   Plasmid

   #73215

   Purpose

   Bacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

   Insert

   EGFR (EGFR Human, Synthetic, Schistosoma japonicum)
   

   Tags

   10XHis-GST-HRV and The dL5 FAP and Her1 affibody …

   Expression

   Bacterial

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   T7

   Available Since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET20b-His6-LONP1-Walker B mutant (E591A)

    Plasmid

    #247938

    Purpose

    Expression of human LONP1 with methionine 115 as its first residue (mature mitochondrial-matrix localized form) and Walker B mutant (E591A)

    Depositor

    Gabriel Lander

    Article

    Shin et al Nat Commun. 2021 May 28;12(1):3239. doi:

    Insert

    LONP1 (LONP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    encodes aa 115-959, with E591A

    Promoter

    T7

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid

    #246982

    Purpose

    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.

    Depositor

    Amro Hamdoun

    Insert

    φC31(PhiC31)
    

    Use

    Synthetic Biology

    Tags

    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-His6-SUMO-Lyn-SH2-AviTag

    Plasmid

    #214207

    Purpose

    Bacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    Lyn A (LYN Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-His6-SUMO-Yes-SH2-AviTag

    Plasmid

    #214208

    Purpose

    Bacterial expression of SH2 domain of the Yes kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    Yes kinase SH2 domain (YES1 Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-His6-SUMO-Fyn-SH2-AviTag

    Plasmid

    #214202

    Purpose

    Bacterial expression of SH2 domain of the Fyn kinase (B- iso) with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    FYN kinase (FYN Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-His6-SUMO-Lck-SH2-AviTag

    Plasmid

    #214203

    Purpose

    Bacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    Lck kinase SH2 domain (LCK Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-His6-SUMO-Blk-SH2-AviTag

    Plasmid

    #214204

    Purpose

    Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    Blk kinase SH2 domain (BLK Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-His6-SUMO-Fgr-SH2-AviTag

    Plasmid

    #214205

    Purpose

    Bacterial expression of SH2 domain of the Fgr kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    Fgr kinase SH2 domain (FGR Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-His6-SUMO-Hck-SH2-AviTag

    Plasmid

    #214206

    Purpose

    Bacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    p60-HCK (HCK Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28 MBP-TEV-Rat BCKDK-His

    Plasmid

    #232119

    Purpose

    For bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    BCKDK (Bckdk Rat)
    

    Tags

    6 x His, Maltose-Binding Protein (MBP), and Tev S…

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-Fgß-FIVAR-ELP-HIS-ybbR

    Plasmid

    #169136

    Purpose

    E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    FIVAR (found in various architectures)
    

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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