162,651 results

1. EGFP-p65

   Plasmid

   #111190

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

   Insert

   RELA (RELA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSCV-Cre

   Plasmid

   #24064

   Purpose

   Retroviral coexpression of Cre and EGFP

   Depositor

   Dan Littman

   Insert

   Cre
   

   Use

   Cre/Lox and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   MESV 5' LTR

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-His:dCas9:nls:10P3

   Plasmid

   #135982

   Purpose

   Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   dCas9 fused to 10 copies of the P3 coiled-coil
   

   Use

   CRISPR

   Tags

   His

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-p58

   Plasmid

   #166942

   Purpose

   mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

   Insert

   p58 (Lman1 Rat)
   

   Use

   Tags

   GFP and chicken lysozyme signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. DsRed-Rab5 DN

   Plasmid

   #13051

   Depositor

   Richard Pagano

   Article

   Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

   Insert

   RAB5A (Canis familiaris) (RAB5A Canis familiaris)
   

   Use

   Tags

   DsRed2 and HA

   Expression

   Mammalian

   Mutation

   asparagine 3 changed to serine (neutral); aspara…

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGWB511

   Plasmid

   #74853

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE EF1α dCas9-NED

   Plasmid

   #109369

   Purpose

   Lentiviral vector for expressing effector domains fused to dCas9.

   Depositor

   Antal Kiss

   Article

   Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

   Plasmid

   #107270

   Purpose

   Expresses Cas9 upon induction with doxicycline.

   Depositor

   Pablo Menéndez

   Article

   Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

   Inserts

   hSpCas9
   rtTA
   bGH polyA-SV40polyA
   

   Use

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TagBFP2-FKBP-PIP5K1C_Kina-HD

   Plasmid

   #202741

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mTagBFP2:FKBP1A(3-108):[GGSA]4GG:PIP5K1C(79-366)(D101R/R304D) (PIP5K1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TagBFP2-FKBP-PIP5K1C_Kina-HDD316K

    Plasmid

    #202742

    Purpose

    expresses recruitable fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:FKBP1A (3-108) : [GGSA]4GG : PIP5K1C(79-366) (D101R / R304D / D316K) (PIP5K1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge