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Salsa-BC271-P3G7

Plasmid

#203112

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC271

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC271

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN2_EF1a-tNGFR-2A-Puro

Plasmid

#201112

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 2

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T48-L432G-Cterminal-mEmerald

Plasmid

#202373

Purpose

Talin(DeltaR2-10)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR2-10)-L432G-mEmerald

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T58-L432G-Cterminal-mEmerald

Plasmid

#202372

Purpose

Talin(DeltaR4-10)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR4-10)-L432G-mEmerald

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T100-L432G-Cterminal-mEmerald

Plasmid

#202371

Purpose

Talin(FullLength)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(FullLength)-L432G-mEmerald

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T46-L432G-Cterminal-mEmerald

Plasmid

#202374

Purpose

Talin(DeltaR4-12)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR4-12)-L432G-mEmerald

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLipoP1-NLS-3xmCitrine

Plasmid

#192522

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

Putative membrane lipoprotein (AT3G18250 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

LipoP1

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-tdTomato-WPRE

Plasmid

#187112

Purpose

FLP-dependent expression of tdTomato under EF1a promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

tdTomato

Use

AAV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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hsa-miR 19b plix 4.03

Plasmid

#121126

Purpose

inducible expression in cells

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-miR-19b

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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hsa-miR199 plix 4.03

Plasmid

#121129

Purpose

inducible expression in cells

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-miR-199

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 141-dendra2

Plasmid

#97112

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 141 dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 R19A

Plasmid

#121112

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (R19A) (CBX8 Human)

Use

Lentiviral

Tags

V5

Mutation

R19A

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p5Etmem88aE1

Plasmid

#90112

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of tmem88a

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

tmem88aE1 (tmem88a Zebrafish)

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pJK130

Plasmid

#71129

Purpose

Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide synthetase

Tags

His6

Expression

Bacterial

Mutation

changed valine-319 to phenylalanine

Promoter

T7

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_LAMP1

Plasmid

#65685

Purpose

Expresses LAMP1-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pYNL-N1_LAMP1

Plasmid

#65684

Purpose

Expresses LAMP1-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pYNL-N1_PTS2

Plasmid

#65675

Purpose

Expresses PTS2-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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SPLICS PM-ER Short P2A

Plasmid

#164112

Purpose

Detect the short Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pST1374-N-NLS-flag-linker-Cas9-H840A

Plasmid

#51129

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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PGK 4xMito-mEmerald

Plasmid

#200430

Purpose

Mammalian expression of fluorescently-tagged marker for mitochondria

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence

Tags

mEmerald

Expression

Mammalian

Mutation

4x mito-targeting sequence

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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