We narrowed to 2,348 results for: 1186

1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. EC71171

   Plasmid

   #154069

   Purpose

   Level 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backbone

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   CRE_U5intron
   

   Use

   Synthetic Biology

   Mutation

   All BsaI, Esp3I and BPiI restriction sites were r…

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AE-Polyubiquitin-GCaMP6s

   Plasmid

   #106868

   Purpose

   Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

   Depositor

   Omar Akbari

   Article

   Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

   Insert

   AE-Polyubiquitin-GCAaMP6s
   

   Expression

   Insect

   Promoter

   Ae. aegypti polyubiquitin (PUb)

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FEV_pet28a

   Plasmid

   #131668

   Purpose

   Bacterial expression of ETS gene FEV

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   FEV (FEV Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HR-TERT-promotor

   Plasmid

   #71398

   Purpose

   Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Promoter

   Endogenous TERT promotor

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC304

   Plasmid

   #118897

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with TEVprotease and TwinStrep-MB…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. amilGFP chromoprotein promoter-less

   Plasmid

   #118006

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, amilGFP
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. ETV1_pet28a

   Plasmid

   #131644

   Purpose

   Bacterial expression of ETS gene ETV1

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETV1 (ETV1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ETS2_pet28a

   Plasmid

   #131664

   Purpose

   Bacterial expression of ETS gene ETS2

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETS2 (ETS2 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRW004-SM-destination-proUBQ10-Cas9

   Plasmid

   #104438

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Plant

   Promoter

   UBQ10

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid

    #98041

    Purpose

    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    Sa-dCas9-NLS-3xFLAG
    

    Use

    CRISPR

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    D10A + H557A

    Promoter

    CMV

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. ELF4_pet28a

    Plasmid

    #131661

    Purpose

    Bacterial expression of ETS gene ELF4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF4 (ELF4 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF005-sgRNA-shuffle-in

    Plasmid

    #104441

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF004-sgRNA-shuffle-in

    Plasmid

    #104440

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    U6 promoter

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW-OVOL2-HA

    Plasmid

    #184737

    Purpose

    Doxycycline-inducible lentiviral expression of OVOL2

    Depositor

    Gloria Manzotti, Alessia Ciarrocchi

    Article

    Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

    Insert

    ovo like zinc finger 2 (OVOL2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR P2R-P3-ECFP

    Plasmid

    #48353

    Purpose

    Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Mutation

    Contains a stop codon.

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPICZ-pre-pro-alphaf-E2-Crimson

    Plasmid

    #117658

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-pro-alphaf-E2-Crimson
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. tsPurple chromoprotein promoter-less

    Plasmid

    #118007

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ230-RRVQ

    Plasmid

    #200188

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RRVQ
    

    Use

    CRISPR; Gateway compatible lbcas12a-rrv entry clo…

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, D156R, R182V, E795Q

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ230-RVQ

    Plasmid

    #200187

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RVQ
    

    Use

    CRISPR; Gateway compatible lbcas12a-rv entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, R182V, E795Q

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR P4-P1R-EYFP

    Plasmid

    #48350

    Purpose

    Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EYFP
    

    Use

    Gateway entry vector

    Mutation

    Contains a Kozak sequence. Does not contain a sto…

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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